PMID: 6168723Sep 1, 1981Paper

Idiotypic repertoire of anti-hen eggwhite lysozyme antibodies probed with hybridomas. Selection after immunization of an IdX marker common to antibodies of distinct epitope specificity

The Journal of Experimental Medicine
D W MetzgerE E Sercarz

Abstract

A panel of hybridoma antibodies obtained from lymphoid cells that were fused during a primary response ("early") or a secondary response ("late") gave results concordant with analysis of conventional, in vivo-produced anti-lysozyme idiotypes: early antibodies did not display the predominant anti-hen eggwhite lysozyme idiotype (IdX-HEL), whereas late antibodies all displayed IdX-HEL. Furthermore, individual late hybridomas could each remove the entire anti-IdX-HEL activity by absorption, whereas early hybridomas could not. The epitope specificities of the hybridomas in both the early and late populations were heterogenous. We conclude that epitypic specificity in the response to HEL is determined independently from idiotypic specificity and that the predominant idiotype is selected for during the maturation of the anti-lysozyme response.

References

Jan 1, 1977·Immunological Reviews·O Mäkelä, K Karjalainen
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F L OwenA Nisonoff
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C A JanewayH Wigzell
Dec 1, 1978·The Journal of Experimental Medicine·R KarolR W Noble
Dec 1, 1979·Molecular Immunology·E EnghoferM J Bosma
Mar 1, 1977·Somatic Cell Genetics·M L GefterM D Scharff
Aug 1, 1980·Springer Seminars in Immunopathology·E E Sercarz, D W Metzger
Jul 1, 1980·The Journal of Experimental Medicine·S T JuM E Dorf

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Citations

Jan 1, 1983·Springer Seminars in Immunopathology·I M RoittP M Lydyard
Feb 1, 1984·The Journal of Clinical Investigation·A Saxon, E Barnett
Sep 17, 2004·International Reviews of Immunology·Radmila Metlas, Veljko Veljkovic
Jan 1, 1983·Annals of the New York Academy of Sciences·A MillerR Riblet
Nov 1, 1983·European Journal of Immunology·D MaleI Roitt
May 19, 2000·Methods : a Companion to Methods in Enzymology·K YamakamiM D Boyle

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