iDISCO+ for the Study of Neuroimmune Architecture of the Rat Auditory Brainstem

Frontiers in Neuroanatomy
Paola PerinRoberto Pizzala

Abstract

The lower stations of the auditory system display a complex anatomy. The inner ear labyrinth is composed of several interconnecting membranous structures encased in cavities of the temporal bone, and the cerebellopontine angle contains fragile structures such as meningeal folds, the choroid plexus (CP), and highly variable vascular formations. For this reason, most histological studies of the auditory system have either focused on the inner ear or the CNS by physically detaching the temporal bone from the brainstem. However, several studies of neuroimmune interactions have pinpointed the importance of structures such as meninges and CP; in the auditory system, an immune function has also been suggested for inner ear structures such as the endolymphatic duct (ED) and sac. All these structures are thin, fragile, and have complex 3D shapes. In order to study the immune cell populations located on these structures and their relevance to the inner ear and auditory brainstem in health and disease, we obtained a clarified-decalcified preparation of the rat hindbrain still attached to the intact temporal bone. This preparation may be immunolabeled using a clearing protocol (based on iDISCO+) to show location and functional state of imm...Continue Reading

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Citations

Oct 7, 2020·Brain Sciences·Agnieszka J SzczepekAlina Smorodchenko
Dec 18, 2020·Frontiers in Neurology·Paola PerinCinzia Boselli
Mar 15, 2021·Journal of Biophotonics·Yanjing ZhanShiwen Zhang
May 1, 2021·Life·Kathrin MalfeldVerena Scheper
May 23, 2021·Nature Protocols·Kurt R WeissJan Huisken
Jul 23, 2021·Progress in Biophysics and Molecular Biology·Pablo Delgado-RodriguezArrate Muñoz-Barrutia
Aug 17, 2021·Frontiers in Cell and Developmental Biology·Paola PerinRoberto Pizzala
Oct 2, 2021·Development·Alba Vieites-Prado, Nicolas Renier

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Methods Mentioned

BETA
imaging techniques

Software Mentioned

ITK
SNAP
FIJI
Fire LUT

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