Abstract
The lower stations of the auditory system display a complex anatomy. The inner ear labyrinth is composed of several interconnecting membranous structures encased in cavities of the temporal bone, and the cerebellopontine angle contains fragile structures such as meningeal folds, the choroid plexus (CP), and highly variable vascular formations. For this reason, most histological studies of the auditory system have either focused on the inner ear or the CNS by physically detaching the temporal bone from the brainstem. However, several studies of neuroimmune interactions have pinpointed the importance of structures such as meninges and CP; in the auditory system, an immune function has also been suggested for inner ear structures such as the endolymphatic duct (ED) and sac. All these structures are thin, fragile, and have complex 3D shapes. In order to study the immune cell populations located on these structures and their relevance to the inner ear and auditory brainstem in health and disease, we obtained a clarified-decalcified preparation of the rat hindbrain still attached to the intact temporal bone. This preparation may be immunolabeled using a clearing protocol (based on iDISCO+) to show location and functional state of imm...Continue Reading
References
Apr 1, 1990·The Annals of Otology, Rhinology, and Laryngology·H N KimI H Chung
Sep 1, 1985·Acta Oto-laryngologica·L I Terr, B J Edgerton
Mar 21, 2006·NeuroImage·Paul A YushkevichGuido Gerig
Feb 17, 2010·Cerebrospinal Fluid Research·Jan G Veening, Henk P Barendregt
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Oct 11, 2012·The Journal of Comparative Neurology·Michael A MuniakDavid K Ryugo
Jan 10, 2013·Molecular Pharmaceutics·N Strazielle, J F Ghersi-Egea
Mar 13, 2013·Immunity·Ravid ShechterMichal Schwartz
Jan 8, 2015·Frontiers in Pharmacology·Masato FujiokaKaoru Ogawa
Dec 18, 2015·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·R Shane TubbsAaron A Cohen-Gadol
Mar 5, 2016·Cell Chemical Biology·Etsuo A Susaki, Hiroki R Ueda
May 31, 2016·Cell·Nicolas RenierMarc Tessier-Lavigne
Jun 18, 2016·Scientific Reports·Marzena StefaniukLeszek Kaczmarek
Jul 19, 2016·Cell Reports·Thomas LiebmannMarc Flajolet
Aug 6, 2016·Cephalalgia : an International Journal of Headache·Jun ZhaoDan Levy
Aug 23, 2016·Neurobiology of Disease·Fernanda MarquesJoana Almeida Palha
Mar 11, 2017·Frontiers in Neuroanatomy·Verónica Fuentes-SantamaríaJosé M Juiz
Mar 23, 2017·Nature Immunology·Marco PrinzNora Hagemeyer
Jul 25, 2017·Frontiers in Cellular Neuroscience·Gilda M KalinecFederico Kalinec
Jul 30, 2017·Journal of Neuroinflammation·Ruimin GeZaal Kokaia
Sep 2, 2017·The Journal of Clinical Investigation·Antoine LouveauJonathan Kipnis
Dec 27, 2017·Hearing Research·Alec N Salt, Keiko Hirose
Citations
Jun 28, 2019·Frontiers in Neurology·Silvia T ErniStephen L Leib
Oct 7, 2020·Brain Sciences·Agnieszka J SzczepekAlina Smorodchenko
Dec 18, 2020·Frontiers in Neurology·Paola PerinCinzia Boselli
Mar 15, 2021·Journal of Biophotonics·Yanjing ZhanShiwen Zhang
May 1, 2021·Life·Kathrin MalfeldVerena Scheper
May 23, 2021·Nature Protocols·Kurt R WeissJan Huisken
Jul 23, 2021·Progress in Biophysics and Molecular Biology·Pablo Delgado-RodriguezArrate Muñoz-Barrutia
Aug 17, 2021·Frontiers in Cell and Developmental Biology·Paola PerinRoberto Pizzala
Oct 2, 2021·Development·Alba Vieites-Prado, Nicolas Renier