PMID: 11925460Apr 2, 2002Paper

IEC-6 cells are an appropriate model of intestinal iron absorption in rats

The Journal of Nutrition
C Thomas, P S Oates

Abstract

Regulation of iron absorption, which is the primary mechanism for maintaining body iron stores, occurs primarily in the proximal small intestine. Recent identification of proteins that are involved in iron absorption such as the uptake transporter-divalent metal transporter (DMT1), the basolateral transporter, IREG1, and the ferroxidase-hephaestin provide new opportunities to study this process. We evaluated the rat intestinal cell line, IEC-6, as a model of intestinal iron transport. This involved measuring the expression of DMT1 and IREG1 by Western blot analysis and confocal microscopy, and hephaestin by protein-dependent copper oxidase activity. DMT1 and IREG1 were expressed in IEC-6 cells. The uptake of 1 micromol/L ferrous iron [Fe(II)]:ascorbate and its efflux also was associated with the expression of DMT1 under different levels of iron loading. The expression of DMT1 changed inversely with iron levels as did the uptake of Fe(II). However, with different levels of cellular iron, IREG1 expression remained constant, as did the release of iron from the cells, suggesting that they could be related. Ceruloplasmin and apotransferrin did not enhance the rate or extent of iron release. Copper oxidase activity, considered to ind...Continue Reading

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Citations

Dec 18, 2009·Journal of Cellular and Molecular Medicine·Natascha A WolffCraig P Smith
May 12, 2018·International Journal of Food Sciences and Nutrition·Yasumi HorimotoLoong-Tak Lim
Aug 15, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·James F CollinsP N Ranganathan
Nov 24, 2012·American Journal of Physiology. Cell Physiology·Liwei Xie, James F Collins
Dec 17, 2015·Journal of Animal Physiology and Animal Nutrition·H ZhangS Bai
May 3, 2007·Pflügers Archiv : European journal of physiology·Phillip S Oates
Aug 3, 2005·Pflügers Archiv : European journal of physiology·Phillip S Oates, Carla Thomas
Aug 28, 2020·Metallomics : Integrated Biometal Science·Haoxuan DingJie Feng
Jul 16, 2016·International Journal of Molecular Sciences·Jeffrey L PlattMarilia Cascalho
Aug 12, 2008·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Roberta J WardKarl Summer
May 23, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Carla Thomas, Phillip S Oates
May 27, 2005·Free Radical Biology & Medicine·Bodiga VijayalakshhmiManchala Raghunath
Sep 4, 2021·Molecular Nutrition & Food Research·Gary Ro-Lin ChangChuan-Mu Chen

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