IFAT and ELISA phase I/phase II as tools for the identification of Q fever chronic milk shedders in cattle

Veterinary Microbiology
Laura LuccheseAlda Natale

Abstract

Q fever is a widespread zoonotic disease caused by Coxiella burnetii. In cattle the bacterial shedding can persist without symptoms for several months and the shedders identification is a critical issue in the control of the infection at herd level. Following the example of the human protocols for the assessment of Q fever infection status, the aim of this study was the evaluation of the antibody response dynamics to phase I and phase II antigens in C. burnetii shedder dairy cows by means of a phase-specific serology, to verify the suitability of the investigated tools in recognising milk shedders. A total of 99 cows were monitored during time and classified on the basis of serological and PCR results in five groups identifying different shedding patterns. The 297 sera collected in three sampling times were tested by means of ELISA IgG for differential phase I and phase II antibodies detection, while a selection of 107 sera were tested by means of phase specific IgM and IgG IFAT. Both ELISA IgG and IFAT IgG highlighted a low reactivity in non-shedder seropositive animals compared to chronic milk shedder animals. ELISA IgG seemed to perform better than IFAT IgG-IgM, showing significant serological differences among groups that a...Continue Reading

References

May 29, 2007·Veterinary Microbiology·Elodie RoussetMichel Aubert
Nov 18, 2008·Applied and Environmental Microbiology·Elodie RoussetAnnie Rodolakis
Jun 23, 2009·Annals of the New York Academy of Sciences·Annie Rodolakis
Oct 31, 2009·Veterinary Microbiology·Emmanouil Angelakis, Didier Raoult
Nov 16, 2012·PloS One·Hendrik-Jan RoestLucien van Keulen
Aug 7, 2013·Veterinary Research·Hendrik I J RoestJohanna M J Rebel

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Citations

Jan 6, 2016·Canadian Journal of Microbiology·Samira BoarbiMarcella Mori
Aug 21, 2016·International Journal of Food Microbiology·Alessia GalieroDomenico Cerri

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