IFNα -Expressing Amniotic Fluid-Derived Mesenchymal Stem Cells Migrate to and Suppress HeLa Cell-Derived Tumors in a Mouse Model

Stem Cells International
Jun ZhouGuangmei Zhang

Abstract

Immunotherapy for cervical cancer with type I interferon (IFN) is limited because of the cytotoxicity that accompanies the high doses that are administered. In this study, we investigated the utilization of amniotic fluid-derived mesenchymal stem cells (AF-MSCs) as a means for delivering IFNα to local tumor sites for the suppression of cervical cancer in a mouse model using HeLa cell xenografts. The tumor tropism ability of AF-MSCs and AF-MSCs genetically modified to overexpress IFNα (IFNα-AF-MSCs) was examined through Transwell in vitro and through fluorescent images and immunohistochemistry in a mouse model. Tumor size and tumor apoptosis were observed to evaluate the efficacy of the targeting therapy. Mechanistically, tumor cell apoptosis was detected by cytometry and TUNEL, and oncogenic proteins c-Myc, p53, and Bcl-2 as well as microvessel density were detected by immunohistochemistry. In this model, intravenously injected AF-MSCs selectively migrated to the tumor sites, participated in tumor construction, and promoted tumor growth. After being genetically modified to overexpress IFNα, the IFNα-AF-MSCs maintained their tumor tropism but could significantly suppress tumor growth. The restrictive efficacy of IFNα-AF-MSCs was...Continue Reading

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Citations

Aug 15, 2018·International Journal of Molecular Sciences·Yi-Heng LinYen-Hua Huang
Feb 14, 2021·Stem Cell Research & Therapy·Ameneh JafariHassan Niknejad
Mar 7, 2021·International Journal of Molecular Sciences·Qun WangHelene Hildegard Heidegger

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Methods Mentioned

BETA
flow cytometry
ELISA
confocal microscopy
light microscopy
fluorescence microscopy
Xenografts
xenograft

Software Mentioned

Pro Plus ( IPP )
Image
Pro Plus (

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