IFN-γ Regulates the Expression of MICA in Human Corneal Epithelium Through miRNA4448 and NFκB

Frontiers in Immunology
Dan WuJiaxu Hong

Abstract

Major histocompatibility complex class I-related chain A (MICA), a non-classical major histocompatibility complex molecule, can stimulate or co-stimulate CD8+ T cells or natural killer (nk) cells, thus affecting cornea allograft survival. This study investigated IFN-γ regulation of MICA expression levels in human corneal epithelium by miRNA4448. MICA expression levels in human corneal epithelial cells (HCECs) stimulated with IFN-γ were detected by qRT-PCR and an enzyme-linked immunosorbent assay, and differential miRNA expression levels were measured. qRT-PCR, Western blotting, and immunofluorescence staining revealed nuclear factor kappa B (NFκB)/P65 expression in IFN-γ-treated and miRNA4448-overexpressed HCECs. A luciferase reporter assay was used to predict the interaction between NFκB and MICA. Additionally, HCECs were transfected with MICA plasmid or treated with IFN-γ and NKG2D-mAb and cocultured with NK cells and CD8+ T cells. Cell apoptosis was measured using Annexin V/PI staining. qRT-PCR detected the expression of anti-apoptosis factor Survivin and apoptosis factor Caspase 3 in MICA-transfected and IFN-γ-treated HCECs after co-culturing with NK cells and CD8+ T cells. IFN-γ (500 ng/ml, 24 h) upregulated MICA expressio...Continue Reading

References

Mar 21, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D StephensonJ Clemens
Oct 3, 2002·Nature Reviews. Immunology·Qiutang Li, Inder M Verma
Oct 21, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Luciana L MolineroNorberto W Zwirner
Aug 18, 2005·The Journal of Biological Chemistry·Carl Y SasakiDan L Longo
Dec 7, 2005·Current Topics in Microbiology and Immunology·S GonzálezT Spies
Jan 5, 2006·Canadian Journal of Physiology and Pharmacology·Zhongjun DongZhigang Tian
Mar 17, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J Y NiederkornE Mayhew
Feb 3, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Philippe SaikaliNathalie Arbour
Jul 7, 2007·Immunology·Anita R Mistry, Chris A O'Callaghan
Nov 6, 2007·Advances in Immunology·Jamie R Schoenborn, Christopher B Wilson
Dec 18, 2008·International Journal of Cancer. Journal International Du Cancer·Nicole SchwinnAnnette Paschen
May 21, 2010·Ocular Immunology and Inflammation·Jerry Y Niederkorn, D Frank P Larkin
Jun 28, 2011·PloS One·Sharon Ka-Wai LeeChi-Pui Pang
Oct 18, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Henrique Borges da SilvaMaria Regina D'Império Lima
Aug 6, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cong WuXuetao Cao
Mar 29, 2016·International Journal of Biological Sciences·Lily BooSoon Keng Cheong

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Citations

Feb 27, 2020·Journal of Clinical Medicine·Antonio Di ZazzoTakenori Inomata
May 1, 2021·Seminars in Cell & Developmental Biology·Saife N LoneMuzafar A Macha

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Methods Mentioned

BETA
flow cytometry
Assay
PCR
transfection
flow-cytometry
enzyme-linked immunosorbent assay
Chip
nuclear translocation
ELISA

Software Mentioned

SPSS
Ingenuity Pathway Analysis ( IPA
miRWalk
Expo32
targetScan

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