IFN-alpha induces MxA gene expression in cultured human corneal fibroblasts

Experimental Eye Research
M E AbramsC R Brandt

Abstract

Treatment of certain human cells with Interferon-alpha (IFN-alpha) induces the synthesis of a 76 kDa protein designated MxA that is involved in resistance to viral infection. We have used a specific cDNA clone and monoclonal Ab to show that MxA is induced in IFN-alpha treated human corneal fibroblast cultures. Mx RNA was increased 23-fold and 45-fold after 5 and 9 h of IFN-alpha treatment, respectively. The MxA protein was detectable by immunoblotting at 5 hr after IFN treatment and peaked at 17 hr. Concentrations of IFN-alpha as low as 1 U/ml induced detectable amounts of MxA, and expression was maximal at 1 x 10(3) U ml-1. These results confirm that MxA synthesis is induced in human corneal fibroblasts treated with IFN-alpha.

References

Jan 1, 1990·Advances in Virus Research·P Staeheli
Aug 1, 1991·Journal of Interferon Research·J Pavlovic, P Staeheli
Sep 1, 1990·Experimental Eye Research·B J TripathiS Raja
Mar 1, 1988·Somatic Cell and Molecular Genetics·M A HorisbergerJ Content
Aug 1, 1993·Journal of Interferon Research·M J BalishC R Brandt

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Citations

Nov 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J L TaylorE C Borden
Jan 23, 1999·Immunological Reviews·J C LeongB Simon
Jul 9, 2004·Developmental and Comparative Immunology·Veronica Bergan, Børre Robertsen
Jun 4, 2021·Communications Biology·Ya-Ling ChangSung-Liang Yu

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