PMID: 2494257Apr 1, 1989Paper

IFN-gamma and IFN-beta independently stimulate the expression of lipopolysaccharide-inducible genes in murine peritoneal macrophages

The Journal of Immunology : Official Journal of the American Association of Immunologists
T A HamiltonC S Tannenbaum

Abstract

We have recently described the isolation and characterization of a set of cDNA encoding genes whose expression is induced or enhanced in murine peritoneal macrophages by treatment with LPS. In the present report we have analyzed the expression of the mRNA which hybridize with these cDNA probes in macrophages treated with other cytokines known to modulate functional activity. Three distinct patterns of expression have been documented. Two genes (D3 and C7) are inducible by LPS, IFN-gamma, and IFN-beta; D3 is comparably sensitive to all three, whereas C7 is more sensitive to LPS and IFN-gamma than to IFN-beta. The mRNA encoded by D8 is expressed in response to LPS and IFN-beta but not in response to IFN-gamma. Finally the gene encoded by D5 is inducible only in cells treated with LPS. The expression of all three cytokine-inducible mRNA was both dose and time dependent and was mediated by increased transcriptional activity of the genes. As with stimulation by LPS, the expression induced by IFN was independent of protein synthesis and occurred in a rapid and transient fashion. TNF-alpha had little or no detectable effect on any of the genes by themselves. The expression of C7, however, could be induced synergistically by treatment ...Continue Reading

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