PMID: 9555982Apr 29, 1998Paper

IFN-gamma induces calcium transients and increases the capacitative calcium entry in human neutrophils

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
V AasJ G Iversen

Abstract

We have previously reported that long-term priming of human polymorphonuclear neutrophilic granulocytes (PMN) with interferon-gamma (IFN-gamma) increased the fMLP-stimulated calcium influx. We now show that also after short-term incubation with IFN-gamma, PMN calcium metabolism is modulated. Single adherent cells in three different calcium-containing buffers (high, normal, and low [Ca2+]) were stimulated with the bacterial peptide fMLP or the Ca-ATPase inhibitor thapsigargin (Tg) after about 5 min preincubation with IFN-gamma. The results of this protocol indicated that IFN-gamma increases both calcium influx and calcium sequestration. Store dependent Ca2+ influx, directly measured on readdition of calcium to Tg-treated cells incubated in EGTA buffer, was significantly enhanced in IFN-gamma-treated cells. This effect of IFN-gamma was enhanced by the tyrosine kinase inhibitor herbimycin A. Strikingly, in low extracellular calcium concentrations, IFN-gamma induced calcium transients in 20%-60% of the cells. The proportion of PMN responding with Ca2+ transients increased with decreasing extracellular calcium concentration. Average lagtime from addition of IFN-gamma to a response that could be measured was 7.3 sec, and average incr...Continue Reading

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J RichterT Andersson
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y KoideT O Yoshida
Nov 15, 1995·The Biochemical Journal·M J Berridge
Jun 1, 1993·Cell Calcium·K Wong, L Kwan-Yeung
Sep 1, 1995·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·V AasJ G Iversen
Nov 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·V AasH B Benestad
May 1, 1997·Journal of Cellular Physiology·J T Mandeville, F R Maxfield

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Citations

Apr 21, 2004·Immunology·Terri N Ellis, Blaine L Beaman
Jan 14, 2003·Biophysical Journal·Lars F OlsenHoward R Petty
Nov 21, 2018·Journal of Thrombosis and Haemostasis : JTH·F-R BertinM D Blostein
Nov 21, 2018·The European Journal of Neuroscience·Saikata SenguptaJulian Grosskreutz
Feb 14, 2019·Journal of Physiology and Biochemistry·Vadim MitrokhinMitko Mladenov

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