IFN-gamma-mediated extravillous trophoblast outgrowth inhibition in first trimester explant culture: a role for insulin-like growth factors

Molecular Human Reproduction
Yuxiang HuPeter von Dadelszen

Abstract

Pre-eclampsia is often associated with inadequate cytotrophoblast invasion and remodelling of the uterine spiral arteries. Examining a first trimester, 2D in vitro explant culture model which mimics in vivo placentation, including trophoblast column formation and extravillous cytotrophoblast (EVT) migration, we previously suggested that excessive maternal decidual natural killer cell interferon (IFN-gamma) limits EVT migration. Types-1 and -2 insulin-like growth factor (IGF-1, IGF-2) are trophic for EVT, act through their surface receptors, IGFR-1 and IGFR-2, and are regulated by the IGF-binding proteins (IGFBPs). Could the observed IFN-gamma-mediated inhibition of EVT outgrowth and migration be related to either expression changes of IGF-1 or IGF-2, their receptors, their binding proteins, or apoptosis? Using the 2D explant culture model, we examined the effect of IFN-gamma exposure on IGF-1 and -2, IGFR-1 and -2, IGFBPs and apoptosis. IFN-gamma relatively increased IGF-1 and -2 secretion. In EVT, IFN-gamma decreased IGFR-2, but not IGFR-1 expression. IGBP-2, -3 and -4 production were not influenced by IFN-gamma. IFN-gamma induced trophoblast apoptosis measured by the highly sensitive M30 neo-epitope, but not caspase 3 activit...Continue Reading

References

Oct 1, 1992·American Journal of Reproductive Immunology : AJRI·J A Hill
Feb 1, 1995·Endocrine Reviews·J I Jones, D R Clemmons
May 1, 1996·Journal of Cellular Physiology·M I Garcia-LloretL J Guilbert
Nov 11, 1998·Nature Medicine·L FinkR M Bohle
Jun 23, 1999·American Journal of Reproductive Immunology : AJRI·S SaitoH Morikawa
Jul 26, 2000·Development, Growth & Differentiation·G M ZouJ Milliez
Dec 18, 2001·International Journal of Cancer. Journal International Du Cancer·B P LeeP K Lala
Feb 28, 2002·American Journal of Respiratory Cell and Molecular Biology·Wayne A PriceAlan D Stiles
Apr 6, 2002·Canadian Journal of Physiology and Pharmacology·Chandan ChakrabortyPeeyush K Lala
Jun 24, 2003·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Jacek R WilczyńskiJan Wilczyński
Sep 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Murry W Wynes, David W H Riches
Nov 18, 2003·Acta Obstetricia Et Gynecologica Scandinavica·Anna HübinetteSven Cnattingius
Jul 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Qing QiuAndrée Gruslin
Dec 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuxiang HuPeter von Dadelszen
Dec 5, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gendie E LashJudith N Bulmer

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Citations

Jan 11, 2011·The Journal of Infectious Diseases·Alexandra J UmbersStephen J Rogerson
Jan 25, 2014·Cell Death & Disease·W LiH Wang
Oct 11, 2012·Experimental and Molecular Pathology·Alexandra KleimanZia A Khan
Jan 6, 2011·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Fatma Ferda Verit, Nese Gul Hilali
Dec 6, 2014·Reproductive Biology and Endocrinology : RB&E·Erin J CrosleyJulian K Christians
Mar 1, 2019·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Xi ChenSuhua Chen
Jul 3, 2009·Biology of Reproduction·Natalie J HannanLois A Salamonsen
Dec 29, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kelly J BainesStephen J Renaud
Jun 13, 2020·Journal of Leukocyte Biology·Soraya Mezouar, Jean-Louis Mege
Nov 17, 2017·Natural Product Research·Le Ba VinhYoung Ho Kim
Feb 16, 2021·Human Immunology·Jianhong Zhang, Stephen J Lye

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