IFN-gamma: regulation of nitric oxide in the P. aeruginosa-infected cornea

Ocular Immunology and Inflammation
Sharon A McClellanLinda Dondero Hazlett

Abstract

BALB/c mice are resistant to Pseudomonas aeruginosa (P. aeruginosa) keratitis and bacterial killing/stasis requires nitric oxide (NO). NO regulation in the cornea is unknown and was tested in this model. Nitrite detection, IFN-gamma-knockout mice, TNF-alpha neutralization, ELISA, aminoguanidine (AG) treatment, MPO, and plate counts were done. Evidence shows (i) without IFN-gamma, nitrite levels are elevated, (ii) neutralization of TNF-alpha does not change nitrite levels, and (iii) absence of IFN-alpha and reduced NO synergistically increases disease progress and upregulates pro-inflammatory cytokines, PMN number, and bacterial load. IFN-gamma regulates NO levels, and synergistic interaction between the two regulates disease outcome in resistant mice.

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Citations

May 15, 2007·Current Opinion in Infectious Diseases·Philip Aloysius Thomas, Pitchairaj Geraldine
Nov 26, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Minhao WuLinda D Hazlett
Apr 30, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Megan E B FoldenauerLinda D Hazlett
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xi HuangRonald P Barrett
Jul 10, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Linda D HazlettRonald P Barrett
Jan 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Minhao WuLinda D Hazlett
May 4, 2021·The Ocular Surface·Shyam Sunder TummanapalliMark D P Willcox

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