PMID: 12759449May 22, 2003Paper

IFN regulatory factor-1 is required for the up-regulation of the CD40-NF-kappa B activator 1 axis during airway inflammation

The Journal of Immunology : Official Journal of the American Association of Immunologists
Zhendong ZhaoXiaoxia Li

Abstract

Recent studies show that NF-kappa B activator 1 (Act1) functions as an important adapter molecule for CD40-mediated signaling in epithelial cells. To explore the physiological function of the CD40-Act1 axis, we studied the regulation of gene expression of CD40 and Act1 both in vivo and in cell culture models. Although CD40 and Act1 are up-regulated in mouse lung upon LPS stimulation, IL-1 plus IFN-alpha, -beta, or -gamma synergistically up-regulate both CD40 and Act1 gene expression in human epithelial A549 cells. Cycloheximide superinduces the Act1 mRNA, whereas actinomycin D completely abolishes the Act1 mRNA, indicating that the induction of Act1 mRNA is at the transcriptional level and does not require protein synthesis. Promoter sequence analyses identified putative IFN regulatory factor (IRF)-1, C/EBP-beta, and AP-1 transcription factor binding sites in the Act1 promoter. Although mutation of any of the three sites abolished the promoter activity, Abs against IRF-1 and C/EBP-beta, but not AP-1, blocked the formation of the DNA-binding complex induced by IL-1 plus IFN-beta stimulation, suggesting cooperative action between IRF-1 and C/EBP-beta in mediating Act1 promoter activity. Importantly, LPS-induced gene expression of...Continue Reading

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Citations

Oct 20, 2007·American Journal of Respiratory Cell and Molecular Biology·Omar TlibaYassine Amrani
May 15, 2009·Clinical and Experimental Immunology·A PeairsC M Reilly
Jan 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shadi SwaidaniXiaoxia Li
Jul 21, 2012·PloS One·Benjamin M J OwensPaul M Kaye
Sep 24, 2016·Pediatric Surgery International·Christian TomuschatPrem Puri
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Francesca CagnoniBruno Azzarone
Oct 20, 2006·Bioinformatics·M Juanita MartinezKevin S Harrod
Oct 16, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Grigory RyzhakovIrina A Udalova
May 5, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ruirui HeChenhui Wang

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