IFNβ signaling inhibits osteogenesis in human SLE bone marrow.

Lupus
Lin GaoRichard John Looney

Abstract

Bone marrow mesenchymal stem cells are multipotent adult stem cells that can differentiate into osteoblasts, adipocytes, and chondrocytes. Our recently published data demonstrate that systemic lupus erythematous bone marrow mesenchymal stem cells produce increased quantities of interferon β based on a positive feedback loop involving the innate signaling molecule mitochondrial antiviral signaling protein. Moreover, this pathway contributes to human systemic lupus erythematous bone marrow mesenchymal stem cell senescence-like features. Here we investigate the differentiation defects of systemic lupus erythematous bone marrow mesenchymal stem cells and the potential for therapeutic interventions. The six systemic lupus erythematous patients recruited in this study satisfy the American College of Rheumatology 1997 classification criteria for systemic lupus erythematous. Systemic Lupus Erythematous Disease Activity Index-2K was used to determine disease activity. Systemic lupus erythematous bone marrow mesenchymal stem cells were isolated with Ficoll centrifugation and phenotyped using flow cytometry. In vitro studies included real-time polymerase chain reaction and western blotting. We compared six age-paired bone marrow aspirates...Continue Reading

References

Nov 1, 1996·The Journal of Clinical Investigation·J J LetterioA B Roberts
Dec 22, 1999·Genes & Development·G Karsenty
Jul 18, 2000·Biochemical Society Transactions·J B LianA J van Wijnen
Oct 14, 2000·Annual Review of Cell and Developmental Biology·B R OlsenW Wang
Dec 16, 2000·Nature·J R Arron, Y Choi
Oct 15, 2003·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Bernard A J Roelen, Peter ten Dijke
Jan 27, 2006·Molecular Biology of the Cell·Olga MoiseevaGerardo Ferbeyre
Mar 13, 2010·Clinical Orthopaedics and Related Research·Wakaba FukushimaYoshio Hirota
Jan 4, 2012·Arthritis Care & Research·Irene E M Bultink
Mar 6, 2013·Biochemia Medica·Janja ZupanJanja Marc
Jan 1, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arumugam PalanichamyJennifer H Anolik
Feb 3, 2015·Journal of Cellular Biochemistry·Oscar DonosoJ Pablo Rodríguez
Feb 18, 2015·Trends in Immunology·Gideon Schreiber, Jacob Piehler
Oct 17, 2015·Arthritis Care & Research·Frances ReesWeiya Zhang

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Citations

Jul 27, 2021·Frontiers in Cell and Developmental Biology·Xinyi LvXiangmei Chen

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Methods Mentioned

BETA
PCR
transfection
Assay
nuclear translocation

Software Mentioned

GraphPad Prism8

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