Ifosfamide-loaded lipid-core-nanocapsules to increase the anticancer efficacy in MG63 osteosarcoma cells

Saudi Journal of Biological Sciences
Sheng-Qun WangGuang-Yao Liu

Abstract

In this study, we mainly aimed at developing the Ifosfamide-loaded-lipid-core nanocapsules (IFS-LNC) to increase the therapeutic efficacy in osteosarcoma. The nanoparticle was prepared and evaluated in terms of physical, chemical and biological parameters. The lipid-core-nanocapsules were nanosized with narrow particle size distribution and exhibited a high loading capacity. The LNC displayed a sustained release profile of the drug suggesting its potential application in biomedical field and prolonged anticancer therapy. The LNC showed an endocytosis-mediated cellular uptake in MG63 cancer cells which may lead to an accelerated disruption of the acidic endolysosomal vesicles with release of IFS into the cytoplasm. Specifically, IFS-LNC exhibited a significantly higher cytotoxicity than free IFS used at the same concentration. The indiscriminate ability of the drug-loaded formulation increased the apoptosis of cancer cells by increasing the expression levels of caspase-3 and caspase-9 in MG63 cells. Overall, nanoparticulate formulations of Ifosfamide enhanced the therapeutic efficacy in osteosarcoma.

Citations

Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Mural QuadrosGunjan Verma
Apr 15, 2021·Biomedical Materials·S Ram PrasadA Jayakrishnan
Oct 12, 2021·Frontiers in Bioengineering and Biotechnology·Hongwei WuGang Huang

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Methods Mentioned

BETA
transmission electron microscopy

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