IgA in the horse: cloning of equine polymeric Ig receptor and J chain and characterization of recombinant forms of equine IgA.

Mucosal Immunology
Melanie J LewisJenny M Woof

Abstract

As in other mammals, immunoglobulin A (IgA) in the horse has a key role in immune defense. To better dissect equine IgA function, we isolated complementary DNA (cDNA) clones for equine J chain and polymeric Ig receptor (pIgR). When coexpressed with equine IgA, equine J chain promoted efficient IgA polymerization. A truncated version of equine pIgR, equivalent to secretory component, bound with nanomolar affinity to recombinant equine and human dimeric IgA but not with monomeric IgA from either species. Searches of the equine genome localized equine J chain and pIgR to chromosomes 3 and 5, respectively, with J chain and pIgR coding sequence distributed across 4 and 11 exons, respectively. Comparisons of transcriptional regulatory sequences suggest that horse and human pIgR expression is controlled through common regulatory mechanisms that are less conserved in rodents. These studies pave the way for full dissection of equine IgA function and open up possibilities for immune-based treatment of equine diseases.

References

Apr 1, 1977·Immunochemistry·K F MitchellD O Morgan
Apr 1, 1985·The Journal of Experimental Medicine·E E Max, S J Korsmeyer
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E E MaxM A Robinson
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L MatsuuchiM E Koshland
Oct 1, 1972·Infection and Immunity·T C McGuire, T B Crawford
Mar 1, 1982·The Journal of Experimental Medicine·M YagiM E Koshland
Sep 1, 1993·Immunology Today·M B MazanecM E Lamm
Apr 9, 1998·Veterinary Immunology and Immunopathology·B WagnerA Radbruch
Feb 17, 1999·The Journal of Experimental Medicine·J M HexhamJ D Capra
Dec 23, 1999·International Immunology·V SørensenI Sandlie
Sep 6, 2000·Scandinavian Journal of Immunology·F E JohansenP Brandtzaeg
Aug 18, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L J MarshallJ K Shute
Mar 21, 2002·Immunogenetics·Julia M Phillips-Quagliata
Jun 20, 2002·Veterinary Immunology and Immunopathology·Duncan Hannant
Nov 22, 2002·FEMS Immunology and Medical Microbiology·Saíd TaoujiClaire Collobert-Laugier
Jan 28, 2003·Trends in Immunology·Armelle Phalipon, Blaise Corthésy
Oct 3, 2003·Veterinary Immunology and Immunopathology·Dorothy M AinsworthDouglas F Antczak
Oct 18, 2003·Immunogenetics·Bettina WagnerDouglas F Antczak
Mar 24, 2004·Trends in Immunology·Finn-Eirik Johansen, Per Brandtzaeg
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
Nov 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melanie J LewisJenny M Woof
Apr 1, 2006·Animal Genetics·M PerrocheauG Guérin
Jan 24, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ranveig BraathenFinn-Eirik Johansen
Dec 7, 2007·Veterinary Immunology and Immunopathology·Bettina WagnerHeather Freer
Mar 22, 2008·Fish & Shellfish Immunology·J H W M RomboutJ J Taverne-Thiele
Jun 14, 2008·Immunity·Andrea Cerutti, Maria Rescigno

❮ Previous
Next ❯

Citations

Apr 2, 2013·Veterinary Immunology and Immunopathology·Alison KegganBettina Wagner
Nov 19, 2014·Equine Veterinary Journal·G A Perkins, B Wagner
Jun 22, 2011·MAbs·Jantine E Bakema, Marjolein van Egmond
Jan 29, 2020·Reproduction, Fertility, and Development·Mariana Diel de AmorimM Anthony Hayes
Oct 4, 2016·Veterinary Immunology and Immunopathology·Anna-Karin E PalmEva Wattrang

❮ Previous
Next ❯

Methods Mentioned

BETA
size
electrophoresis
glycosylation
PCR
enzyme-linked immunosorbent assay
size exclusion chromatography
chip

Software Mentioned

BIAevaluation
ClustalW
MEGA3

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.