IgE binding activities and in silico epitope prediction of Der f 32 in Dermatophagoides farinae

Immunology Letters
Yuwei LiZhigang Liu

Abstract

Dermatophagoides farinae is a common indoor allergen source that produces more than 30 allergens, which induces diverse allergic diseases such as allergic rhinitis, allergic asthma and atopic dermatitis. Der f 32 is an inorganic pyrophosphatase and an important allergen from Dermatophagoides farinae. In the present study, Der f 32 was cloned, expressed and purified in order to better understand its structure and immunogenicity. Immunoblotting analysis and ELISA showed 5 of 5 positive reactions to recombinant Der f 32 using serum from house dust mite (HDM)-allergic patients. We constructed homology modeling and predicted epitopes of Der f 32 via bioinformatic tools. The sequence and structural analysis indicated that Der f 32 belonged to the pyrophosphatase family and represented a special structure of external α-helices and internal antiparallel closed β-sheets. In addition, eight B-cell epitopes and four T-cell epitopes were predicted. B-cell epitopes were 24-31, 111-121, 135-140, 168-172, 200-207, 214-220, 237-243, and 268-274 and T-cell epitopes were 47-55, 78-90, 127-135 and 143-151. The B-cell epitopes were distributed completely on the surface of Der f 32 and were located largely in random coils of secondary structures. H...Continue Reading

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