IgE-binding to vicilin-like antimicrobial peptides is associated with systemic reactions to macadamia nut

Clinical and Translational Allergy
Anna M EhlersAndré C Knulst


Macadamia nut can induce fatal allergic reactions and changes in dietary habits will raise their consumption in industrialised countries. Until now diagnosis of macadamia nut allergy by sIgE solely relies on the macadamia nut extract, but single components are lacking. Macadamia nut proteins recognised by IgE from 2 macadamia nut extract positive sera were identified by mass spectrometry (vicilin-like antimicrobial peptides: VLAP). Sensitisation to macadamia nut extract and heterologously expressed isoform VLAP-2-3 was evaluated in 82 nut allergic (NA) and 27 tolerant (NT) patients (no tree nut allergy reported) comprehending 10 macadamia nut allergic (MA) and 18 explicitly reported macadamia nut tolerant patients (MT), using line blots. Co-sensitisation to additional VLAP isoforms and other vicilins was evaluated in 8 MA, 12 MT and 14 NA patients sensitised to VLAP-2-3. Functional properties were determined by indirect basophil activation. Even though proteins recognised by IgE were identified as VLAP-2-1, 2-2 and 2-3, only peptides specifically belonging to VLAP-2-3 were detected by mass spectrometry. The macadamia nut extract was recognised by 33% of NA patients (27/82) including 3 MA patients and 26% of NT patients (7/27, 3...Continue Reading


Oct 16, 1999·The Journal of Allergy and Clinical Immunology·M F SutherlandC Suphioglu
Oct 14, 2004·The Journal of Allergy and Clinical Immunology·Elide A PastorelloClaudio Ortolani
Mar 27, 2010·Journal of the European Academy of Dermatology and Venereology : JEADV·R A HerbstP J Frosch
Aug 2, 2017·The Journal of Allergy and Clinical Immunology·Mari SasakiKatrina J Allen

❮ Previous
Next ❯


Jun 8, 2021·Allergology International : Official Journal of the Japanese Society of Allergology·Nobuyuki Maruyama

❮ Previous
Next ❯

Methods Mentioned


Related Concepts

Related Feeds

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Related Papers

Journal of the European Academy of Dermatology and Venereology : JEADV
E KnottU Darsow
Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology
D E Pallares
© 2021 Meta ULC. All rights reserved