PMID: 7518296Apr 1, 1994Paper

IgE-receptor activated chloride uptake in relation to histamine secretion from rat mast cells

British Journal of Pharmacology
U G FriisJ C Foreman

Abstract

1. Antigen-stimulated histamine secretion from rat peritoneal mast cells was inhibited when extracellular chloride was replaced by either isethionate or gluconate anions, but the histamine release still remained quite substantial. 2. Rat peritoneal mast cells take up 36Cl and the uptake reaches a steady state after 60 min incubation with the isotope. At steady state, the intracellular chloride level in the cells was calculated to be 29 +/- 11.5 mM. 3. The chloride uptake in mast cells was exponential with a rate constant of 0.036 min-1 in resting cells. When the cells were stimulated with antigen, and rate constant for chloride uptake increased to 0.90 min-1: an increase of 25 fold. Under identical experimental conditions histamine release increased 3 fold. 4. The rate of chloride uptake in either resting cells or in antigen-stimulated cells was not changed when the extracellular medium was nominally calcium-free but histamine release was almost completely inhibited in the absence of extracellular calcium. 5. The putative chloride channel blocker DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid) 0.3 to 30 microM, produced a concentration-related inhibition of antigen-stimulated histamine secretion but DIDS (30 microM) ...Continue Reading

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Citations

Oct 14, 2009·European Biophysics Journal : EBJ·Lina WangWolfgang Schwarz
Aug 1, 1996·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·M CalongeJ C Pastor
Aug 23, 2001·International Immunopharmacology·P ForsytheA D Befus
Mar 22, 2002·British Journal of Pharmacology·Valeria SpeltaR Alan North
Apr 23, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·I Ashmole, P Bradding
Jan 6, 2010·Journal of Cellular Physiology·Henrique B SilvaJosé H M Nascimento
Aug 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P J BarnesR Pauwels
Jul 9, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P Bradding, E C Conley
Jun 1, 1996·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A A Norris
Mar 1, 1996·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A A Norris, E W Alton
Jul 30, 2005·The Journal of Pharmacology and Experimental Therapeutics·Marianna KulkaA Dean Befus
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S M DuffyP Bradding

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