IGF-II/mannose-6-phosphate receptor signaling induced cell hypertrophy and atrial natriuretic peptide/BNP expression via Galphaq interaction and protein kinase C-alpha/CaMKII activation in H9c2 cardiomyoblast cells

The Journal of Endocrinology
Chun-Hsien ChuChih-Yang Huang

Abstract

The role played by IGF-II in signal transduction through the IGF-II/mannose-6-phosphate receptor (IGF2R) in heart tissue has been poorly understood. In our previous studies, we detected an increased expression of IGF-II and IGF2R in cardiomyocytes that had undergone pathological hypertrophy. We hypothesized that after binding with IGF-II, IGF2R may trigger intracellular signaling cascades involved in the progression of pathologically cardiac hypertrophy. In this study, we used immunohistochemical analysis of the human cardiovascular tissue array to detect expression of IGF2R. In our study of H9c2 cardiomyoblast cell cultures, we used the rhodamine phalloidin staining to measure the cell hypertrophy and western blot to measure the expression of cardiac hypertrophy markers atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) in cells treated with IGF-II. We found that a significant association between IGF2R overexpression and myocardial infarction. The treatment of H9c2 cardiomyoblast cells with IGF-II not only induced cell hypertrophy but also increased the protein level of ANP and BNP. Using Leu27IGF-II, an analog of IGF-II which interacts selectively with the IGF2R, to specifically activate IGF2R signaling casc...Continue Reading

Citations

Jul 11, 2012·Clinical and Experimental Pharmacology & Physiology·Kimberley C W WangJanna L Morrison
Apr 14, 2012·Circulation Research·Luigi FontanaValter D Longo
Nov 30, 2013·TheScientificWorldJournal·Jacqueline Heger, Klaus-Dieter Schlüter
Sep 27, 2014·BMC Complementary and Alternative Medicine·Chih-Yang HuangDennis Jine-Yuan Hsieh
Nov 28, 2015·International Journal of Molecular Sciences·Wei-Kung ChenChih-Yang Huang
Dec 6, 2011·Growth Factors·Lynda K Harris, Melissa Westwood
Feb 20, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yueh-Shan WengChih-Yang Huang
Feb 22, 2017·The Journal of Endocrinology·Kimberley C W WangJanna L Morrison
Dec 5, 2017·Journal of Diabetes Research·O EscribanoA Gómez-Hernández
Jun 15, 2017·BioMedicine·Marthandam Asokan ShibuChih-Yang Huang
Dec 24, 2014·Journal of Cellular Biochemistry·Kuan-Ho LinChih-Yang Huang
Aug 30, 2012·The Journal of Physiology·Kimberley C W WangJanna L Morrison
Jan 30, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kimberley C W WangJanna L Morrison
Dec 22, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Finny MonickarajArup Das
Oct 9, 2019·Scientific Reports·Nikolay Vadimovich KukushkinThomas James Carew
Aug 17, 2017·Journal of Receptor and Signal Transduction Research·Ya-Fang ChenChih-Yang Huang
Apr 19, 2016·Immunological Reviews·Mohammed S ChaudhryMarcel R M van den Brink
Jun 13, 2014·Journal of the American Heart Association·Maryam Sharifi-SanjaniJeffrey S Isenberg
Sep 21, 2010·Molecular and Cellular Biochemistry·Yi-Jiun WengJin-Ming Hwang

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