IGF2 role in adrenocortical carcinoma biology

Endocrine
Sofia S PereiraDuarte Pignatelli

Abstract

Clinical outcomes of adrenocortical carcinomas (ACC) could be improved by using novel treatment targets based on the recent advances of tumor biology knowledge. Insulin-like growth factor 2 (IGF2) protein expression is usually 8-80 fold higher in ACC when compared to normal adrenal glands (N-AG) or adrenocortical adenomas (ACA), despite the fact that the biological features of high vs. low IGF2 expressing ACC have not yet been well characterized. Our goal was to understand the IGF2 role in ACC biology by focusing in several cancer hallmarks, including cell proliferation, viability, invasion, and metabolism. IGF2 immunohistochemistry expression was evaluated in ACC (n = 13), non-functioning adrenocortical adenoma (ACAn) (n = 14), and N-AG (n = 9). The effects of IGF2 (50, 100 ng/mL) in cell proliferation, viability, invasion, and metabolism, as well as in MAPK/ERK and mTOR pathways activation and N-cadherin expression, were evaluated in the ACC human cell line H295R. IGF2 expression was increased in ACC compared to ACAn and N-AG. Exposure to 100 ng/mL of IGF2 increased H295R cell proliferation and viability. mTOR inhibition reverted IGF2 triggered cell proliferation and viability while MEK/MAPK/ERK inhibition only reverted IGF2 ...Continue Reading

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Citations

Aug 13, 2020·Cancers·Marthe ChehadeStan Sidhu
Apr 21, 2021·Der Pathologe·Wolfgang Saeger
Jul 16, 2021·Rare Tumors·Rui Caetano OliveiraMaria Augusta Cipriano
Jul 22, 2021·European Journal of Drug Metabolism and Pharmacokinetics·Malik Salman HaiderRobert Luxenhofer
May 15, 2021·Journal of Molecular Cell Biology·Weiwei GuiHong Li

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Methods Mentioned

BETA
Protein Assay
electrophoresis
ELISA

Software Mentioned

GraphPad Prism
[UNK]
Quantity One
European Network for
ImageJ
ENSAT
GraphPad

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