IGF2BP1 over-expression in skin squamous cell carcinoma cells is essential for cell growth

Biochemical and Biophysical Research Communications
Zhengjun LiuTian-Lan Zhao

Abstract

The present study examined expression and potential functions of insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) in human skin squamous cell carcinoma (SCC). We show that IGF2BP1 mRNA and protein expression levels were upregulated in established (A431 line) and primary human skin SCC cells. Its expression was also increased in human skin SCC tissues, as compared to the normal skin tissues. In skin SCC cells, IGF2BP1 silencing or CRISPR/Cas9 knockout decreased levels of IGF2BP1-stablized mRNAs, including IGF2, CD44, Gli1 and Myc. Furthermore, skin SCC cell survival and proliferation were inhibited by IGF2BP1 silencing/knockout. Conversely, forced over-expression of IGF2BP1 further promoted A431 cell survival and proliferation. Furthermore, siRNA-mediated knockdown of IGF2BP1-bound long non-coding RNA THOR ("Lnc-THOR") similarly depleted IGF2BP1-dependent mRNAs, causing inhibition on A431 cell survival and proliferation. In vivo, IGF2BP1 silencing or knockout inhibited A431 tumor xenograft growth in mice. Together, we conclude that IGF2BP1 over-expression in skin SCC cells is essential for cell growth.

Citations

Nov 20, 2018·Molecular Oncology·Lotteke J Y M SwierJoost Kluiver
Jul 18, 2020·Journal of Clinical Medicine·Andreea D LazarMarieta Costache
Sep 13, 2020·Cell Death & Disease·Jian ZhangLong Cheng
Mar 19, 2020·Frontiers in Oncology·Yujia WangXian Jiang
Jun 19, 2019·International Journal of Molecular Sciences·Jiahui ChenWen Luo
Mar 27, 2021·Frontiers in Cell and Developmental Biology·Soudeh Ghafouri-FardMohammad Taheri
Apr 20, 2019·Biochemical and Biophysical Research Communications·Zheng-Wei LiHai-Hui Qin
Jul 13, 2021·Investigational New Drugs·Qiu-Ying DuDong-Sheng Pei
Sep 22, 2021·Animal Science Journal = Nihon Chikusan Gakkaihō·Xiaoli XuJiaxue Cao
Dec 7, 2021·Frontiers in Oncology·Peng-Fei JiaoGao-Fei Ren

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