Igf2bp3 maintains maternal RNA stability and ensures early embryo development in zebrafish.

Communications Biology
Fan RenJie Mei

Abstract

Early embryogenesis relies on maternally inherited mRNAs. Although the mechanism of maternal mRNA degradation during maternal-to-zygotic transition (MZT) has been extensively studied in vertebrates, how the embryos maintain maternal mRNA stability remains unclear. Here, we identify Igf2bp3 as an important regulator of maternal mRNA stability in zebrafish. Depletion of maternal igf2bp3 destabilizes maternal mRNAs prior to MZT and leads to severe developmental defects, including abnormal cytoskeleton organization and cell division. However, the process of oogenesis and the expression levels of maternal mRNAs in unfertilized eggs are normal in maternal igf2bp3 mutants. Gene ontology analysis revealed that these functions are largely mediated by Igf2bp3-bound mRNAs. Indeed, Igf2bp3 depletion destabilizes while its overexpression enhances its targeting maternal mRNAs. Interestingly, igf2bp3 overexpression in wild-type embryos also causes a developmental delay. Altogether, these findings highlight an important function of Igf2bp3 in controlling early zebrafish embryogenesis by binding and regulating the stability of maternal mRNAs.

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Citations

Aug 31, 2020·Molecular Reproduction and Development·Daniel ŻarskiJulien Bobe
Aug 28, 2020·Frontiers in Cell and Developmental Biology·Meng ZhangZiyi Li
Jul 31, 2020·Nature·Eric L Van NostrandGene W Yeo
Apr 14, 2020·Current Pharmaceutical Design·Peng-Fei WangShu-Lin Liu
Jul 18, 2021·Cell Biology and Toxicology·Peizhe SongGuifang Jia
Aug 18, 2021·The Journal of Experimental Medicine·Amalie C GrenovZiv Shulman

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Methods Mentioned

BETA
gene knockout
immunoprecipitation
RNA-seq
PCR
genotyping
in vitro transcription
RIP
RIP-seq

Software Mentioned

LAS
STAR
featureCounts
R
LAS X
GraphPad Prism
clusterProfiler
fastp
RSEM
edgeR Bioconductor

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