IGFBP-6 plays a role as an oncosuppressor gene in NPC pathogenesis through regulating EGR-1 expression

The Journal of Pathology
Yuan-Sung KuoChin-Tarng Lin

Abstract

Nasopharyngeal carcinoma (NPC) is prevalent in south-eastern Asia, particularly southern China, Singapore and Taiwan. The aim of this study was to identify the pivotal genes that may be altered during NPC progression. Using cDNA microarray analysis, we compared the expression of 18 genes between NPC and normal nasomucosal cells. qRT-PCR analysis found the expression of IBFBP-6 in NPC cell lines and immunolocalization of IGFBP-6 in NPC to be very weak. To explore the effects of IGFBP-6 on NPC tumour growth, we constructed inducible plasmids containing full-length IGFBP-6 cDNA (pBIG2i-IGFBP-6) and established pBIG2i-IGFBP-6-transfected NPC stable cell lines (NPC-TW01-pBIG2i-IGFBP-6). We then performed functional analysis of the IGFBP-6 in cell lines in vitro and in vivo. Over-expression of IGFBP-6 significantly suppressed the proliferation, invasion and metastatic activity of NPC cells and increased their apoptosis. We found the EGR-1, caspase-1 and TSP-1 genes to be markedly up-regulated when NPC-pBIG2i-IGFBP-6 was treated with doxycycline. Knocking down EGR-1 with EGR-1 siRNA resulted in a decrease in expression of caspase-1, TSP-1 and EGR-1 but not the expression of IGFBP-6. However, in knockdown cells the unchanged expression...Continue Reading

References

Jan 1, 1990·Journal of Cancer Research and Clinical Oncology·R J TatakeS G Gangal
Aug 1, 1989·British Journal of Cancer·M C YuB E Henderson
Feb 15, 1982·International Journal of Cancer. Journal International Du Cancer·Y ZengG R Su
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·C LiuD Mercola
Jul 3, 1997·International Journal of Cancer. Journal International Du Cancer·R P HuangE D Adamson
Dec 16, 1998·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·V IlvesmäkiR Voutilainen
Sep 4, 1999·Occupational and Environmental Medicine·C K HoC T Lin
Dec 18, 2001·International Journal of Cancer. Journal International Du Cancer·M A GallicchioL A Bach
Mar 5, 2002·The Journal of Endocrinology·P GrellierS Babajko
Jun 18, 2002·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Eileen D Adamson, Dan Mercola
Jun 17, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Han-Chung WuChin-Tarng Lin
Jan 9, 2004·Cancer Cell International·Antonella CalogeroGiuseppe Ragona
Sep 28, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Han-Chung WuChin-Tarng Lin
May 26, 2005·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Leon A Bach
Jul 22, 2006·International Journal of Cancer. Journal International Du Cancer·Wei ShiFei-Fei Liu
Sep 22, 2006·Functional & Integrative Genomics·Yuan-Chii Gladys LeeChi-Ying F Huang
Dec 6, 2008·The EMBO Journal·Jianxiu YuTomas Mustelin
Jun 23, 2009·The American Journal of Pathology·Yu-Chyi HwangChin-Tarng Lin

❮ Previous
Next ❯

Citations

Apr 12, 2012·Nutrition and Cancer·Seethalakshmi SreenivasanSubramanian Krishnakumar
Nov 5, 2013·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·A KonermannW Götz
Apr 13, 2011·Journal of Cell Communication and Signaling·Andrea TafernerHaymo Pircher
Sep 6, 2011·Journal of the American Society of Hypertension : JASH·Richard N Re, Julia L Cook
Nov 3, 2015·Best Practice & Research. Clinical Endocrinology & Metabolism·Leon A Bach
May 28, 2011·International Journal of Cancer. Journal International Du Cancer·Chunyang ZhangCunming Duan
Nov 22, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Xiao-Lu ZhuYin-Cheng Teng
Nov 7, 2012·Clinical Science·Leon A BachZhiyong Yang
Apr 22, 2014·The Tohoku Journal of Experimental Medicine·Yong XuXu Zhang
Mar 27, 2015·Journal of Cell Communication and Signaling·Leon A Bach
Dec 2, 2014·International Journal of Oncology·Xiaochen LiMin Xie
Aug 9, 2011·Mechanisms of Ageing and Development·Lucia MicutkovaPidder Jansen-Duerr
Sep 30, 2016·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Leon A Bach
Dec 20, 2017·Journal of Molecular Endocrinology·L A Bach
Aug 18, 2018·Journal of Cellular and Molecular Medicine·Arcangelo LisoMassimo Conese
Sep 14, 2016·Oncotarget·Qiuyan ChenYi Lu
Jun 13, 2020·Journal of Cellular Physiology·Zhecun WangShenming Wang
Mar 28, 2018·Bulletin of Experimental Biology and Medicine·S V NikulinM Yu Shkurnikov
Jun 22, 2021·Frontiers in Genetics·Sergey NikulinAlexander Tonevitsky

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis