IGFBP6 regulates vascular smooth muscle cell proliferation and morphology via cyclin E-CDK2.

Journal of Cellular Physiology
Zhecun WangShenming Wang

Abstract

Despite the high prevalence of varicose veins, the underlying pathogenesis of this disease remains unclear. The present study aims to explore the role of insulin-like growth factor binding protein 6 (IGFBP6) in vascular smooth muscle cells (VSMCs). Using a protein array approach, we identified several differentially expressed proteins between varicose great saphenous veins and normal great saphenous veins. Bioinformatic analysis showed that IGFBP6 was closely related to cell proliferation. Further validation confirmed that IGFBP6 was one of the most highly expressed proteins in varicose vein tissue. Knocking down IGFBP6 in VSMCs significantly attenuated cell proliferation and induced the S phase arrest during the cell cycle. Further experiments demonstrated that IGFBP6 knockdown increased cyclin E ubiquitination, which reduced expression of cyclin E and phosphorylation of CDK2. Furthermore, IGFBP6 knockdown arrested centrosome replication, which subsequently influenced VSMC morphology. Ultimately, IGFBP6 was validated to be involved in VSMC proliferation in varicose vein tissues. The present study reveals that IGFBP6 is closely correlated with VSMC biological function and provides unprecedented insights into the underlying path...Continue Reading

References

Oct 11, 2003·Molecular Cell·Markus WelckerJames M Roberts
Sep 15, 2004·The Journal of Biological Chemistry·Xin YeJ Wade Harper
Apr 20, 2005·Oncogene·Harry C Hwang, Bruce E Clurman
Apr 20, 2005·Oncogene·David Cobrinik
Oct 31, 2006·The Journal of Surgical Research·Joseph J NaoumChangyi Chen
Oct 23, 2009·The British Journal of Surgery·C S Lim, A H Davies
Feb 26, 2010·Cell Division·C Elizabeth Caldon, Elizabeth A Musgrove
Dec 22, 2011·Cell Cycle·Ka Tat SiuAlex C Minella
Nov 7, 2012·Clinical Science·Leon A BachZhiyong Yang
Mar 27, 2013·Phlebology·J D Raffetto
Apr 30, 2013·Biochimica Et Biophysica Acta·Christina RaykhaDavid B O'Gorman
Jul 24, 2013·Nature Reviews. Molecular Cell Biology·Cosetta BertoliRobertus A M de Bruin
May 8, 2014·Cancer Research·Lakshmi GopinathanPhilipp Kaldis
Feb 4, 2015·Cold Spring Harbor Perspectives in Biology·Jingyan FuDavid M Glover
Mar 27, 2015·Journal of Cell Communication and Signaling·Leon A Bach
Jan 26, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Sumi SurendranChandrasekharan C Kartha
Sep 27, 2016·Current Opinion in Cell Biology·Ariana D Sanchez, Jessica L Feldman
Sep 30, 2016·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Leon A Bach
Oct 7, 2016·Science·Arun KanakkantharaJan M van Deursen
Aug 19, 2017·Journal of Vascular Surgery. Venous and Lymphatic Disorders·Yongbo XuHaibo Chu
Apr 19, 2019·PLoS Genetics·Alexandra S ShadrinaYakov A Tsepilov

❮ Previous
Next ❯

Citations

Aug 17, 2021·Neurological Research·Zhitao ZongHua Guo

❮ Previous
Next ❯

Related Concepts

Related Feeds