PMID: 7538331Jan 1, 1994Paper

IgG+, CD5+ human chronic lymphocytic leukemia B cells. Production of IgG antibodies that exhibit diminished autoreactivity and IgG subclass skewing

Autoimmunity
M WakaiMariella Dono

Abstract

Several questions exist regarding CD5+ B cells. These include the ability of these cells, as compared to CD5- B cells, to undergo an Ig isotype class switch, the subclasses utilized, and the effects that switching may have on antigen binding. To address these issues, ten patients with chronic lymphocytic leukemia (CLL) whose CD5+ leukemic B cell clones produced IgG were studied. Monoclonal IgG was collected from PMA-stimulated CLL cells and from heterohybridomas constructed with these cells, and then analyzed for IgG subclass utilization, autoreactivity, and DNA idiotype expression. The monoclonal B cells from 80% of the CLL patients produced IgG1 and those from 20% produced IgG3. None produced IgG2. In contrast to the known autoreactivity of IgM-producing CD5+ CLL cells (> 50% autoreactive), none of these IgG antibodies reacted significantly with the autoantigens tested. However, three did react significantly with autoantigen after artificially increasing antibody valency by crosslinking. Whereas five of the IgG molecules expressed a cross reactive idiotypic (CRI) marker characteristic of non-mutated kappa anti-DNA antibodies, three expressed a CRI displayed primarily on mutated IgG anti-DNA antibodies. Thus, some CD5+ human B...Continue Reading

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Citations

Dec 4, 2009·Nature Reviews. Cancer·Thorsten ZenzStephan Stilgenbauer
Mar 5, 2003·Annual Review of Immunology·Nicholas Chiorazzi, Manlio Ferrarini
Oct 1, 1996·The Journal of Clinical Investigation·F FaisN Chiorazzi
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Aug 4, 2005·Blood·Kostas StamatopoulosAthanasios Fassas
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Apr 20, 2018·Frontiers in Immunology·Walaa DarwicheHussein Ghamlouch
Mar 23, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kathleen N PotterFreda K Stevenson

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