PMID: 25797348DOI: 10.1016/j.ab.2015.03.012Mar 24, 2015Paper
IgG subclass specificity to C1q determined by surface plasmon resonance using Protein L capture technique
Analytical Biochemistry
Rekha PatelBruce A Andrien
Abstract
Recombinant monoclonal antibodies (mAbs) have become an important category of biological therapeutics. mAbs share the same structures and biological functions as endogenous IgG molecules. One function is complement-dependent cytotoxicity (CDC) initiation by binding of C1q. Traditionally, ELISA methods have been utilized to measure C1q binding. A new robust capture method was established in this study to measure the binding affinity of C1q to antibodies by surface plasmon resonance (SPR). The utility of this method was demonstrated by determination of the difference in IgG subclass specificity of C1q binding.
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Jul 20, 2019·PloS One·Maryam M BhattiJan-Willem Theunissen
Aug 26, 2019·Biologicals : Journal of the International Association of Biological Standardization·Milan Jovic, Florian Cymer
Mar 3, 2021·Analytica Chimica Acta·Meral YüceYılmaz Çapan
Mar 27, 2018·Analytical Biochemistry·Wei ZhouYali Li
Sep 4, 2021·Communications Biology·Joshua M SoppMark S Cragg
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