IgM, IgG, and IgA anti-DNA topoisomerase I antibodies in systemic sclerosis.

Journal of Clinical Laboratory Analysis
Erasmo Martínez-CorderoDiana E Aguilar León

Abstract

Anti-DNA topoisomerase I (anti-topo I) antibodies have been broadly studied in systemic sclerosis (SSc). The use of different native and molecularly cloned antigens has shown a predominant IgG response, and a variable frequency of positive IgM and IgA tests. We report herein the serological findings of SSc using a recombinant topo I obtained through a standard bacterial system. Anti-topo I antibodies were studied in 45 SSc patients and 85 healthy controls through ELISA and western blot. Escherichia coli XL1-blue strain and pT7-7 vector were used to amplify a DNA topo I cDNA clone, and to obtain the recombinant polypeptide. The latter was purified by affinity chromatography, and the enzymatic and antigenic properties were evaluated through specific tests. A native antigen was included for comparison. The SSc group disclosed positive IgM (20%), IgG (86.6%), and IgA (26.6%) anti-topo I tests with the recombinant polypeptide, and a purified calf thymus antigen yielded similar results. IgG autoantibodies were frequently associated with skin involvement, esophageal dysfunction, and restrictive lung disease. The recombinant protein showed a molecular weight of 86.6 kDa, a positive topo I activity using a supercoiled pBR322 DNA relaxat...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 1, 1992·Arthritis and Rheumatism·N RothfieldB Greenberg
Sep 1, 1992·Clinical and Experimental Immunology·R VerheijenW J van Venrooij
Apr 1, 1990·Clinical and Experimental Immunology·R VerheijenW J van Venrooij
Feb 1, 1992·Arthritis and Rheumatism·J D ReveilleF C Arnett
Feb 1, 1990·The British Journal of Dermatology·M Jarzabek-ChorzelskaG G Maul
May 1, 1990·Arthritis and Rheumatism·S HildebrandtN F Rothfield
Dec 1, 1990·Clinical Immunology and Immunopathology·S HildebrandtN F Rothfield
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G G MaulS A Jimenez
Oct 1, 1986·The British Journal of Dermatology·M Jarzabek-ChorzelskaE H Beutner
Jun 25, 1985·Journal of Immunological Methods·C Geisler, M Høier-Madsen
Feb 1, 1986·Seminars in Arthritis and Rheumatism·A Alonso-RuizJ Beltrán-Gutiérrez
Mar 1, 1985·The Journal of Clinical Investigation·W J van VenrooijL B van de Putte
Oct 1, 1984·European Journal of Biochemistry·B SchmittH P Vosberg
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L F Liu, K G Miller
Feb 1, 1983·Annals of the Rheumatic Diseases·L J CatoggioP J Maddison
Mar 1, 1995·Clinical Immunology and Immunopathology·D Vázquez-AbadN F Rothfield
Jan 1, 1993·Rheumatology International·S HildebrandtH H Peter
Mar 7, 2001·Arthritis and Rheumatism·H A GussinM Teodorescu
Jul 27, 2002·Clinical and Experimental Medicine·Aguilar D E LeónE Martínez-Cordero
Oct 22, 2003·Current Opinion in Rheumatology·Michelle L Harris, Antony Rosen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.