IgM polymerization inhibits the Golgi-mediated processing of the mu-chain carboxy-terminal glycans

Molecular Immunology
M M CalsRoberto Sitia

Abstract

Secreted glycoproteins generally contain oligosaccharides of the complex type. However, several molecules have been described in which individual glycans are processed differently from one another. Folding, assembly and oligomerization could affect the maturation of certain glycans by hindering them to the Golgi processing machinery. We have tested this possibility by analysing a panel of engineered murine mu chains secreted as mu2L2 monomers or as polymers, and having or not the carboxy-terminal glycan (Asn563). In secreted IgM polymers, Asn563 bears high-mannose oligosaccharides, typical of endoplasmic reticulum resident proteins, while complex sugars are found at the other four sites (Brenckle and Kornfeld, 1980 Arch. Biochem. Biophys. 243, 605-618). Polymeric and monomeric IgM contain mu chains whose glycans are processed differently. We show here that this is mainly due to the differential processing at the Asn563 glycan, which undergoes Golgi-mediated processing when IgM are secreted in the monomeric form. These results indicate that the oligomerization-dependent accessibility to the sugar modifying enzymes can be one of the key features that dictate the extent of oligosaccharide processing in multimeric glycoproteins. Th...Continue Reading

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Citations

Jul 14, 2001·Molecular Immunology·M J ColomaS L Morrison
Nov 5, 2010·Diabetes, Obesity & Metabolism·M Cortini, R Sitia
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Andreas LoosHerta Steinkellner
Aug 1, 2015·Scientific Reports·Maria Francesca MossutoRoberto Sitia
Apr 3, 2016·Journal of the American Society for Mass Spectrometry·Edward S X MohNicolle H Packer
Apr 5, 2002·Fish & Shellfish Immunology·Bergljót MagnadóttirPauline M Rudd
Mar 10, 2018·Expert Review of Proteomics·Arun V Everest-DassNicolle H Packer
Oct 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Dzana PasalicJohannes Buchner
Oct 18, 1996·The Journal of Biological Chemistry·C IsidoroR Sitia
May 27, 2005·The Journal of Biological Chemistry·Regina KuliawatPeter Arvan
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael K Pangburn
Apr 26, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M K PangburnA K Sharma
Sep 18, 2007·The Journal of General Virology·Mireille de Turenne-Tessier, Tadamasa Ooka
Jun 1, 2006·Journal of Cell Science·Laura MattioliCaterina Valetti
Nov 29, 2007·Journal of Cell Science·Ilana ShapiraShoshana Bar-Nun
Feb 9, 2011·Developmental and Comparative Immunology·Jianmin YeStephen Kaattari

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