Ih current is necessary to maintain normal dopamine fluctuations and sleep consolidation in Drosophila.

PloS One
Alicia Gonzalo-GomezInmaculada Canal

Abstract

HCN channels are becoming pharmacological targets mainly in cardiac diseases. But apart from their well-known role in heart pacemaking, these channels are widely expressed in the nervous system where they contribute to the neuron firing pattern. Consequently, abolishing Ih current might have detrimental consequences in a big repertoire of behavioral traits. Several studies in mammals have identified the Ih current as an important determinant of the firing activity of dopaminergic neurons, and recent evidences link alterations in this current to various dopamine-related disorders. We used the model organism Drosophila melanogaster to investigate how lack of Ih current affects dopamine levels and the behavioral consequences in the sleep:activity pattern. Unlike mammals, in Drosophila there is only one gene encoding HCN channels. We generated a deficiency of the DmIh core gene region and measured, by HPLC, levels of dopamine. Our data demonstrate daily variations of dopamine in wild-type fly heads. Lack of Ih current dramatically alters dopamine pattern, but different mechanisms seem to operate during light and dark conditions. Behaviorally, DmIh mutant flies display alterations in the rest:activity pattern, and altered circadian ...Continue Reading

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Citations

Nov 7, 2014·PloS One·Abhishek Chadha, Boaz Cook
Dec 3, 2016·Heart Failure Reviews·Shasha ZhuWuzhou Yuan
Dec 14, 2019·Genes & Development·Maricela Robles-MurguiaFabio Demontis
Mar 14, 2017·Frontiers in Molecular Neuroscience·Andrew P HegleEric A Accili
Jun 25, 2019·Frontiers in Molecular Neuroscience·Xiaoli ChangJunxia Xie
Mar 16, 2016·G3 : Genes - Genomes - Genetics·Darren J ParkerMaaria Kankare
Dec 20, 2018·International Journal of Molecular Sciences·Federica De LazzariFederica Sandrelli
Apr 20, 2021·G3 : Genes - Genomes - Genetics·Jamilla Akhund-ZadeBenjamin L de Bivort

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Methods Mentioned

BETA
PCR
fluorescence-activated cell sorting
Fluorescence Activated Cell Sorting
transgenic
fluorescence
FACS

Software Mentioned

ImageJ
SPSS13
MATLAB
Clocklab
Actimetrics
FlySiesta
SPSS

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