PMID: 9630232Jun 18, 1998Paper

IHF modulation of Tn10 transposition: sensory transduction of supercoiling status via a proposed protein/DNA molecular spring

Cell
R M ChalmersN Kleckner

Abstract

Architectural protein IHF modulates Tn10 transposition in vitro. IHF stimulates transposon excision. Also, separately, IHF forces transposon end/target DNA interactions into a constrained pathway, "channeling," that yields only unknotted intratransposon inversion circles. Negative supercoiling influences both effects, differently. We infer that IHF is an architectural catalyst: it promotes initial transpososome assembly and is then ejected from the transpososome. IHF then rebinds, altering transpososome conformation to promote channeling. We also infer that the developing transpososome is a molecular spring: DNA provides basic elasticity; a conformational change in transposase provides force; and IHF and/or supercoiling provide conformational inputs. In vivo, IHF is a sensory transducer of chromosomal supercoiling status: with supercoiling absent, IHF is "supercoiling relief factor"; with supercoiling present, stimulation and channeling comprise a homeostatic pair such that modest changes in chromosome condition strongly influence transpositional outcome.

References

Oct 1, 1991·Current Opinion in Genetics & Development·R C Johnson
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·H S KooL F Liu
Sep 1, 1991·Genes & Development·I P MoskowitzR C Johnson
Oct 20, 1989·Cell·H W Benjamin, N Kleckner
Oct 9, 1987·Cell·D Morisato, N Kleckner
Mar 20, 1987·Journal of Molecular Biology·J B Bliska, N R Cozzarelli
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D E Pettijohn, O Pfenninger
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·M S Lee, R Craigie
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·Z Wang, R M Harshey
Jan 1, 1996·Current Topics in Microbiology and Immunology·N KlecknerS Bolland
Feb 16, 1996·Journal of Molecular Biology·K R BenjaminN R Cozzarelli
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·M CarmonaB Magasanik
Mar 1, 1990·Genetics·N Kleckner

❮ Previous
Next ❯

Citations

Aug 4, 2009·Genetica·Corentin Claeys Bouuaert, Ronald M Chalmers
Jan 20, 2004·Journal of Molecular Biology·Brandon Ason, William S Reznikoff
Jun 23, 2004·Research in Microbiology·Zita Nagy, Michael Chandler
Jun 8, 2002·Journal of Molecular Biology·John S Allingham, David B Haniford
Apr 20, 2010·Molecular Biology and Evolution·Daniel M Stoebel, Charles J Dorman
Nov 25, 2003·Nucleic Acids Research·Ruy JáureguiEnrique Merino
Aug 22, 2009·Nucleic Acids Research·Simon J WardleDavid B Haniford
Jan 8, 2013·Nucleic Acids Research·Mihai CiubotaruDavid G Schatz
Sep 17, 2005·Genes & Development·Simon J WardleDavid B Haniford
Jul 1, 2008·Journal of Bacteriology·P RousseauM Chandler
Mar 29, 2001·Journal of Bacteriology·Y ShigaE Ohtsubo
Jun 22, 2005·Journal of Bacteriology·Abbie M CorosKeith M Derbyshire
Sep 16, 2004·Molecular and Cellular Biology·Mihai Ciubotaru, David G Schatz
Jan 18, 2012·Journal of Molecular Microbiology and Biotechnology·Zhongge Zhang, Milton H Saier
Nov 28, 2008·BMC Molecular Biology·Ludivine SinzelleCorinne Augé-Gouillou
Mar 15, 2014·PLoS Genetics·Yongming WangZsuzsanna Izsvák
Jul 14, 2009·PloS One·Julien Bischerour, Ronald Chalmers
Feb 24, 2012·PloS One·Lukasz DziewitDariusz Bartosik
Feb 26, 2016·Journal of the American Chemical Society·Yoshimasa SuzukiEiji Yashima
Mar 24, 2006·Molecular Microbiology·Sally-J RowlandW Marshall Stark
Nov 30, 2011·Protein Science : a Publication of the Protein Society·Min LiRobert Craigie
Feb 17, 2005·Journal of Molecular Biology·Saima HumayunDavid B Haniford
Jan 25, 2005·Journal of Molecular Biology·Geyi LiuPerry B Hackett
Aug 18, 2009·Molecular Microbiology·Zhongge Zhang, Milton H Saier
Nov 18, 2003·Annual Review of Genetics·Finbarr Hayes

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.