IHF stabilizes pathogenicity island I of uropathogenic Escherichia coli strain 536 by attenuating integrase I promoter activity.

Scientific Reports
Marco ChittòUlrich Dobrindt

Abstract

Pathogenicity islands (PAIs) represent horizontally acquired chromosomal regions and encode their cognate integrase, which mediates chromosomal integration and excision of the island. These site-specific recombination reactions have to be tightly controlled to maintain genomic stability, and their directionality depends on accessory proteins. The integration host factor (IHF) and the factor for inversion stimulation (Fis) are often involved in recombinogenic complex formation and controlling the directionality of the recombination reaction. We investigated the role of the accessory host factors IHF and Fis in controlling the stability of six PAIs in uropathogenic Escherichia coli strain 536. By comparing the loss of individual PAIs in the presence or absence of IHF or Fis, we showed that IHF specifically stabilized PAI I536 and that in particular the IHFB subunit seems to be important for this function. We employed complex genetic studies to address the role of IHF in PAI I536-encoded integrase (IntI) expression. Based on different YFP-reporter constructs and electrophoretic mobility shift assays we demonstrated that IntI acts a strong repressor of its own synthesis, and that IHF binding to the intI promoter region reduces the ...Continue Reading

References

Dec 1, 1992·Journal of Bacteriology·A M Campbell
Sep 1, 1992·Molecular Microbiology·M FreundlichP Tsui
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·L Moitoso de Vargas, A Landy
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·B S Parekh, G W Hatfield
Sep 15, 1997·Nucleic Acids Research·D Esposito, J J Scocca
Jan 1, 1997·Plasmid·E Hiszczyńska-Sawicka, J Kur
Feb 10, 1998·Clinical Rheumatology·J P SiebJ Jakschik
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jun 29, 2000·The Journal of Biological Chemistry·S M ArfinG W Hatfield
Feb 15, 2001·The Journal of Infectious Diseases·W E Stamm, S R Norrby
Apr 3, 2001·Current Opinion in Microbiology·S M McLeod, R C Johnson
Oct 6, 2001·Current Opinion in Microbiology·U Dobrindt, J Hacker
May 6, 2003·Journal of Bacteriology·Dominic Esposito, Gary F Gerard
Apr 6, 2004·Virology·Simona CalìDaniela Ghisotti
Apr 22, 2004·Nature Reviews. Microbiology·Ulrich DobrindtJörg Hacker
Sep 3, 2005·Nature Reviews. Microbiology·Laura S FrostAriane Toussaint
Jun 8, 2006·Annual Review of Biochemistry·Nigel D F GrindleyPhoebe A Rice
Jul 19, 2006·The Journal of General Virology·D PiazzollaD Ghisotti
Aug 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Elzbieta BrzuszkiewiczUlrich Dobrindt
Sep 16, 2006·The EMBO Journal·Jeffrey P MummJeff Gelles
Apr 9, 2008·Nature Reviews. Microbiology·Niyaz AhmedSeyed E Hasnain
May 6, 2008·Journal of Bacteriology·Emily StonehouseKaren Skorupski
Jan 24, 2009·Trends in Microbiology·E Fidelma BoydMichelle A Parent
May 7, 2010·Medical Microbiology and Immunology·Ulrich DobrindtJ Hacker
Dec 15, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Michael G NapolitanoE Fidelma Boyd
Feb 19, 2011·Bioinformatics·Charles E GrantWilliam Stafford Noble
Mar 19, 2011·Journal of Medical Microbiology·Ciarán P Kelly, Lorraine Kyne
Mar 16, 2012·The New England Journal of Medicine·Thomas M Hooton

❮ Previous
Next ❯

Citations

Sep 26, 2021·Current Biology : CB·Aisling BradyJosé R Penadés

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
fluorescence microscopy
PCR
electrophoretic mobility shift assay
electrophoretic mobility shift
footprinting
protein assay
Electrophoresis

Software Mentioned

FIMO
BD FACSDiva
MEME

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.