IkappaBgamma is expressed in mast cells

Virchows Archiv : an International Journal of Pathology
Michael G HaaseGustavo B Baretton

Abstract

IkappaBgamma (IkappaBgamma) is a 70-kDa protein that is encoded by the C-terminal part of the NF-kappaB p105 gene and acts as an inhibitor of the transcription factor NF-kappaB. Until now, IkappaBgamma expression has only been described in cell-culture models of B-lymphocytes and enterocytes but not in tissues. In a model of radiation-induced pulmonary damage, we found that mast cells accumulating after irradiation are the only cells in the rat lung that are positive for IkappaBgamma. The mast cells were characterised by their metachromatic staining with toluidine blue and by double immunofluorescence labelling with mast-cell tryptase. Western blotting revealed that the lung mast cells expressed the 70-kDa form of IkappaBgamma cytoplasmatically and that no alternative splicing variants were expressed. In addition, we studied 11 cases of systemic mastocytosis, as well as 5 cases of mast-cell hyperplasia. In all cases, the mast cells stained strongly with IkappaBgamma. Rat peritoneal mast cells also contained high levels of IkappaBgamma. Since NF-kappaB is an important regulator of mast-cell functions, IkappaBgamma is likely to play a central role in the maintenance of the mast-cell phenotype and possibly in the modification of m...Continue Reading

References

May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·R J Grumont, S Gerondakis
Mar 17, 2000·The Journal of Allergy and Clinical Immunology·D L Marquardt, L L Walker
May 18, 2000·International Journal of Radiation Biology·M HaaseM Müller
Jun 18, 2002·International Archives of Allergy and Immunology·Masashi UmedaSusumu Furukawa
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·William R CowardMartin K Church
Dec 31, 2003·International Journal of Radiation Biology·M G HaaseG B Baretton

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Citations

Aug 8, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Michael G HaaseGustavo B Baretton
Feb 21, 2006·Clinical and Experimental Dermatology·Q XiaX J Zhang
Mar 29, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Michael G HaaseGustavo B Baretton

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