IL-1β -Induced Matrix Metalloprotease-1 Promotes Mesenchymal Stem Cell Migration via PAR1 and G-Protein-Coupled Signaling Pathway

Stem Cells International
Ming-Siou ChenHwai-Shi Wang

Abstract

Mesenchymal stem cells (MSCs) are known for homing to sites of injury in response to signals of cellular damage. However, the mechanisms of how cytokines recruit stem cells to target tissue are still unclear. In this study, we found that the proinflammation cytokine interleukin-1β (IL-1β) promotes mesenchymal stem cell migration. The cDNA microarray data show that IL-1β induces matrix metalloproteinase-1 (MMP-1) expression. We then used quantitative real-time PCR and MMP-1 ELISA to verify the results. MMP-1 siRNA transfected MSCs, and MSC pretreatment with IL-1β inhibitor interleukin-1 receptor antagonist (IL-1RA), MMP tissue inhibitor of metalloproteinase 1 (TIMP1), tissue inhibitor of metalloproteinase 2 (TIMP2), MMP-1 inhibitor GM6001, and protease-activated receptor 1 (PAR1) inhibitor SCH79797 confirms that PAR1 protein signaling pathway leads to IL-1β-induced cell migration. In conclusion, IL-1β promotes the secretion of MMP-1, which then activates the PAR1 and G-protein-coupled signal pathways to promote mesenchymal stem cell migration.

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Citations

Sep 8, 2019·International Journal of Molecular Sciences·Renata SzydlakPiotr Laidler
Jun 12, 2020·Stem Cells·Jane L LiesveldOmar S Aljitawi
Apr 4, 2021·International Journal of Molecular Sciences·Silvia Fernández-FrancosFrancisco J Vizoso
Aug 28, 2021·International Journal of Molecular Sciences·Teruo NishidaJunko Murakami
Jan 22, 2022·Journal of Drug Targeting·Negah MahhengamHomayoon Siahmansouri

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Datasets Mentioned

BETA
GM6001

Methods Mentioned

BETA
electrophoresis
chip
PCR
Enzyme-Linked Immunosorbent Assay
ELISA
protein assay
transfection

Software Mentioned

SPSS

Related Concepts

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