IL-1α and IL-1β as alternative biomarkers for risk assessment and the prediction of skin sensitization potency

Journal of Toxicology and Environmental Health. Part a
Min Kook KimByung-Mu Lee

Abstract

Potential biomarkers of skin sensitization in RAW264.7 mouse macrophages were investigated as alternatives to animal experiments and risk assessment. The concentrations that resulted in a cell viability of 90% (CV90) and 75% (CV75) were calculated by using a water-soluble tetrazolium salt (WST)-1 assay and used to analyze the skin sensitization potency of 23 experimental materials under equivalent treatment conditions. In addition, the expression of interleukin (IL)-1α, IL-1β, IL-31, tumor necrosis factor (TNF)-α, inducible nitric oxide synthase (iNOS), prostaglandin E2 (PGE2), and cyclooxygenase-2 (COX-2) was analyzed utilizing Western blotting. In the cell viability analysis, skin sensitizers were generally more cytotoxic and exhibited increased skin sensitization potency. However, nonsensitizers did not show any marked cytotoxic tendency. Biomarker analysis demonstrated that IL-1α, IL-1β, and the combination of IL-1α and IL-1β (IL-1α + IL-1β) predicted reliably skin sensitization potential (1) sensitivities of 94.4%, 83.3%, and 83.3%, specificities of 100%, 100%, and 100%, and (2) accuracies of 95.7%, 87%, and 87%, respectively. These observations correlated most reliably as indicators for skin sensitization potency. Data su...Continue Reading

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Citations

Jan 22, 2019·Journal of Toxicology and Environmental Health. Part a·Byung-Mu LeeKyoungeun Kim
Feb 12, 2019·Journal of Toxicology and Environmental Health. Part a·Byung-Mu LeeKyoungeun Kim
May 16, 2019·Journal of Toxicology and Environmental Health. Part a·Jason Y Y WongNathaniel Rothman
Aug 21, 2019·Regulatory Toxicology and Pharmacology : RTP·Renato Ivan de ÁvilaMarize Campos Valadares
Apr 10, 2021·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Kyu-Bong KimByung-Mu Lee
Jul 2, 2021·Journal of Toxicology and Environmental Health. Part a·Geon Ho KimTae Cheon Jeong
Jan 5, 2022·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Isisdoris Rodrigues de SouzaDaniela Morais Leme

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Methods Mentioned

BETA
protein assay
electrophoresis
enzyme-linked immunosorbent assay

Software Mentioned

ImageJ

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