Abstract
A high density of blood vessels is observed in the perforated disks of temporomandibular joint (TMJ), but the underlying mechanism is unknown. This study aimed to explore the regulation of disk angiogenesis in the perforated disks. Expressions of vascular endothelial growth factor (VEGF), angiogenin-1 (Ang-1), chondromodulin-1 (ChM-1), and thrombospondins-1 (TSP-1) were compared between healthy and perforated TMJ disk cells with or without interleukin-1β (IL-1β) incubation. The tube formation, cell migration, and expressions of matrix-metalloproteinases (MMPs) in human umbilical vein endothelial cell line (HUV-EC-C) were investigated in conditional media of disk cells. Western blot was performed to determine protein level of VEGF, Ang-1, ChM-1 and TSP-1 in IL-1β-induced disk cells cultured by NF-κB- or P38-specific pathway inhibitors, respectively. Conditional media from perforated disk cells induced more tube formation, cell migration, and MMPs' expression in HUV-EC-C. Expressions of VEGF and Ang-1 were significantly higher, and ChM-1 and TSP-1 were lower in perforated disks compared to healthy disks. The VEGFA concentration was 291.1 ± 36.09 pg/ml in perforated disk cell conditioned media, markedly larger than that in NDCCM (...Continue Reading
References
Aug 1, 1991·Experimental Cell Research·B Lyons-GiordanoE C Arner
May 11, 2001·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·R SrinivasT Salo
Mar 22, 2003·Archives of Oral Biology·R LeonardiM Caltabiano
Dec 16, 2004·Archives of Oral Biology·Akihiko YamaguchiShigeyuki Fujita
May 30, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Minako MurataKayo Masuko-Hongo
Jul 13, 2006·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Kan YoshidaRyuuzi Fukuda
Mar 27, 2007·Journal of Dental Research·J KeQ G Meng
Oct 28, 2008·Biochemical and Biophysical Research Communications·Kosuke WatariMayumi Ono
Mar 6, 2010·Dento Maxillo Facial Radiology·X M LiuZ Z Chen
Sep 8, 2010·European Journal of Histochemistry : EJH·N KigaS Fujita
May 10, 2011·Joint, Bone, Spine : Revue Du Rhumatisme·Jung-Yoon ChoeSeong-Kyu Kim
Nov 11, 2011·European Journal of Histochemistry : EJH·N KigaS Fujita
May 4, 2012·Cold Spring Harbor Perspectives in Medicine·Patrick R Lawler, Jack Lawler
Jul 11, 2013·Cytokine & Growth Factor Reviews·Andrew Moss
Aug 13, 2013·Molecular Medicine Reports·Zhan JingJianying Feng
Aug 13, 2013·PloS One·Akinori TokitoShuichi Hamasaki
Mar 1, 2014·Journal of Oral Rehabilitation·K D KristensenT K Pedersen
Mar 29, 2014·Protein Science : a Publication of the Protein Society·Brian KrummJunpeng Deng
Apr 16, 2014·Frontiers in Physiology·Elena VoronovRon N Apte
Jul 30, 2014·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Luis Eduardo AlmeidaPaula C Trevilatto
Aug 19, 2014·Journal of Korean Neurosurgical Society·Joo Han KimYoun Kwan Park
Oct 4, 2014·Osteoarthritis and Cartilage·L H S CevidanesM Styner
Dec 3, 2014·Frontiers in Immunology·Ajay JainEduardo Davila
Dec 24, 2014·Osteoarthritis and Cartilage·M C CornejoD Purmessur
Jan 13, 2015·Osteoarthritis and Cartilage·M C EmbreeJ J Mao
Mar 7, 2015·Journal of Dental Research·X D WangY H Zhou
Sep 13, 2015·Archives of Oral Biology·Shufang LuoWei Fang
Citations
Sep 12, 2018·Orthodontics & Craniofacial Research·Rafaela ScariotAlexandre R Vieira
Aug 30, 2018·American Journal of Physiology. Cell Physiology·Muchen DongQingjun Zhou
Apr 3, 2020·Journal of Cellular and Molecular Medicine·Xia FanXiaoqing Yan
Oct 31, 2017·Journal of Cellular and Molecular Medicine·Yaping FengXing Long
Jul 19, 2019·Cellular and Molecular Life Sciences : CMLS·Sipin ZhuJiake Xu
May 6, 2021·Journal of Cellular and Molecular Medicine·Baochao LiHuang Li
Aug 5, 2021·Scientific Reports·Mian XiaoXing Long