IL-1β-regulating angiogenic factors expression in perforated temporomandibular disk cells via NF-κB pathway

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Jie XuXing Long

Abstract

A high density of blood vessels is observed in the perforated disks of temporomandibular joint (TMJ), but the underlying mechanism is unknown. This study aimed to explore the regulation of disk angiogenesis in the perforated disks. Expressions of vascular endothelial growth factor (VEGF), angiogenin-1 (Ang-1), chondromodulin-1 (ChM-1), and thrombospondins-1 (TSP-1) were compared between healthy and perforated TMJ disk cells with or without interleukin-1β (IL-1β) incubation. The tube formation, cell migration, and expressions of matrix-metalloproteinases (MMPs) in human umbilical vein endothelial cell line (HUV-EC-C) were investigated in conditional media of disk cells. Western blot was performed to determine protein level of VEGF, Ang-1, ChM-1 and TSP-1 in IL-1β-induced disk cells cultured by NF-κB- or P38-specific pathway inhibitors, respectively. Conditional media from perforated disk cells induced more tube formation, cell migration, and MMPs' expression in HUV-EC-C. Expressions of VEGF and Ang-1 were significantly higher, and ChM-1 and TSP-1 were lower in perforated disks compared to healthy disks. The VEGFA concentration was 291.1 ± 36.09 pg/ml in perforated disk cell conditioned media, markedly larger than that in NDCCM (...Continue Reading

References

May 11, 2001·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·R SrinivasT Salo
May 30, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Minako MurataKayo Masuko-Hongo
Jul 13, 2006·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Kan YoshidaRyuuzi Fukuda
Sep 8, 2010·European Journal of Histochemistry : EJH·N KigaS Fujita
May 4, 2012·Cold Spring Harbor Perspectives in Medicine·Patrick R Lawler, Jack Lawler
Mar 1, 2014·Journal of Oral Rehabilitation·K D KristensenT K Pedersen
Mar 29, 2014·Protein Science : a Publication of the Protein Society·Brian KrummJunpeng Deng
Apr 16, 2014·Frontiers in Physiology·Elena VoronovRon N Apte
Jul 30, 2014·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Luis Eduardo AlmeidaPaula C Trevilatto
Mar 7, 2015·Journal of Dental Research·X D WangY H Zhou

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Citations

Sep 12, 2018·Orthodontics & Craniofacial Research·Rafaela ScariotAlexandre R Vieira
Aug 30, 2018·American Journal of Physiology. Cell Physiology·Muchen DongQingjun Zhou
Apr 3, 2020·Journal of Cellular and Molecular Medicine·Xia FanXiaoqing Yan
Oct 31, 2017·Journal of Cellular and Molecular Medicine·Yaping FengXing Long
Jul 19, 2019·Cellular and Molecular Life Sciences : CMLS·Sipin ZhuJiake Xu

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