IL-10 Protects Mice From the Lung Infection of Acinetobacter baumannii and Contributes to Bacterial Clearance by Regulating STAT3-Mediated MARCO Expression in Macrophages.

Frontiers in Immunology
Min-Jung KangJong-Hwan Park

Abstract

Interleukin-10 plays important, yet contrasting, roles in host protection against bacterial infections and in the septic response. To determine the role of IL-10 in the host defense against Acinetobacter baumannii infection, wild-type (WT) and IL-10-deficient mice were infected intranasally with the bacteria. IL-10-deficient mice exhibited increased mortality, severe pathology, and excess production of proinflammatory cytokines and chemokines in the lungs, and increased bacterial burdens in bronchoalveolar lavage (BAL) fluids and lung homogenates after A. baumannii infection, compared to WT mice. Intranasal administration of recombinant IL-10 rescued mice from the lethality of the bacterial infection by promoting bacterial clearance and reducing production of cytokines and chemokines in the lungs. In vitro experiments revealed that IL-10 enhanced phagocytosis and bacterial killing by macrophages by upregulating the macrophage receptor with collagenous structure (MARCO). In addition, A. baumannii-induced activation of STAT3 was impaired in IL-10-deficient macrophages, which was essential for expression of MARCO. Intranasal adoptive transfer of WT macrophages resulted in significant increases in mice survival and bacterial cleara...Continue Reading

References

Jul 1, 1984·The Journal of Experimental Medicine·A CeladaR D Schreiber
Nov 1, 1996·The Journal of Infectious Diseases·T van der PollS F Lowry
Jul 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E M BenkhartH W Ziegler-Heitbrock
Jun 26, 2004·Journal of Leukocyte Biology·Virginia S CarlMichael F Smith
Sep 17, 2005·Immunobiology·Kyung-Hyun Park-MinLionel B Ivashkiv
Oct 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mohamed S ArredouaniLester Kobzik
Feb 1, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Pierre Edouard Fournier, Hervé Richet
Sep 21, 2006·The Journal of Biological Chemistry·Raymond F HamiltonAndrij Holian
Dec 23, 2008·American Journal of Respiratory Cell and Molecular Biology·Lei SunThomas P Shanley
May 8, 2009·Journal of Microbiology and Biotechnology·Eui-Suk JeongYang-Kyu Choi
Jan 1, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniel N WolfeEric T Harvill
Dec 1, 2010·Cytokine & Growth Factor Reviews·Robert SabatJens Geginat
Apr 20, 2011·Microbes and Environments·Hamuel James DoughariSpinney Benade
Oct 13, 2011·IUBMB Life·Gustavo M Cerqueira, Anton Y Peleg
May 10, 2012·FEMS Microbiology Reviews·Michael J McConnellJerónimo Pachón
Dec 1, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael G DorringtonDawn M E Bowdish
Dec 18, 2013·PLoS Pathogens·Sargurunathan SubashchandraboseHarry L T Mobley
Feb 12, 2014·Brain, Behavior, and Immunity·Dan HuDeling Yin
Apr 15, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Muzo WuLester Kobzik

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Citations

Aug 28, 2020·Frontiers in Immunology·Séverine BézieCarole Guillonneau
Oct 13, 2020·Frontiers in Cellular and Infection Microbiology·Wangxue Chen
Mar 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Nan ChiangCharles N Serhan
Jul 1, 2021·Open Forum Infectious Diseases·Shubhanshi TrivediDaniel T Leung

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Methods Mentioned

BETA
lavage
ELISA
PCR
electrophoresis

Software Mentioned

GraphPad Prism
GraphPad

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