IL-10 secretion increases signal persistence of HEMA-MMA-microencapsulated luciferase-modified CHO fibroblasts in mice

Tissue Engineering. Part a
Martin SurzynM V Sefton

Abstract

Microencapsulation of cells in a polymer membrane [e.g., poly(hydroxyethyl methacrylate-co-methyl methacrylate) (HEMA-MMA)] has been proposed as a vehicle for the delivery of therapeutic biomolecules, but cells (especially xenogeneic cells) survive only for short times, limiting the utility of this approach. Murine interleukin-10 (mIL-10) has been shown to downregulate the xenogeneic immune response, and we tested the hypothesis that mIL-10 produced by microencapsulated Chinese hamster ovary (CHO) cells would modulate the transplant-site environment leading to prolonged cell function in a xenogeneic model without other immunomodulatory agents. Prior to encapsulation, CHO cells were genetically engineered to express mIL-10 and a firefly bioluminescence protein, luciferase, which allowed for noninvasive tracking of transplanted cells in vivo with the Xenogen IVIS Imaging System. This nondestructive imaging system was sufficiently sensitive to detect photon signal emitted by a single capsule containing around 800 luciferase-transduced CHO (CHO(LUC)) cells in vitro, and to track changes in luciferase expression in vivo over time. Effective modulation of the transplantation-site environment with mIL-10 secreted from capsules was evi...Continue Reading

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Citations

Dec 20, 2011·Journal of Healthcare Engineering·Wujie Zhang, Xiaoming He
Jul 23, 2014·Journal of Tissue Engineering and Regenerative Medicine·Aubrey R Tiernan, Athanassios Sambanis
Nov 20, 2009·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Anna Aihua LiMurray A Potter
Apr 29, 2014·Journal of Biomedical Materials Research. Part a·Ronke M Olabisi
Feb 29, 2020·Biomaterials Science·Barbara Kupikowska-Stobba, Dorota Lewińska
Jun 20, 2019·Frontiers in Bioengineering and Biotechnology·Shuixan Hu, Paul de Vos

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Methods Mentioned

BETA
bioluminescence imaging
Assay
restriction
electrophoresis
transfection
ELISA
FACS
dissecting
flow cytometry
histone acetylation

Software Mentioned

Living Image Software
Cell Quest

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