IL-10 suppressor activity and ex vivo Tr1 cell function are impaired in multiple sclerosis

European Journal of Immunology
Ivan Martinez-ForeroPablo Villoslada

Abstract

T regulatory cells type 1 (Tr1 cells) are excellent candidates for cell therapy in multiple sclerosis (MS). The aim of our study was to assess the functional state of Tr1 cells and IL-10R signaling in patients with MS. Tr1 cells were induced in vitro by activation with anti-CD46 antibodies in controls and patients with MS. Cells were phenotyped by cytometry and suppression assays, and the expression of cytokines and transcription factors was evaluated by real-time PCR, ELISA, cytometry and Western blotting. We found that the activity of Tr1 cells and IL-10R signaling is impaired in MS patients since Tr1 cells isolated from MS patients produced less IL-10 than those obtained from controls. Indeed, the supernatants from Tr1 cells from controls did not suppress the proliferation of stimulated CD4(+) cells from patients with MS. Furthermore, the IL-10R signaling pathway was not fully active in CD4(+) cells from MS patients and these cells had higher baseline levels of SOCS3 transcripts than controls. Indeed, after in vitro IL-10 stimulation, the expression levels of the STAT1, STAT3 and IL-10RA genes were higher in MS patients than in controls. Moreover, Stat-3 phosphorylation was lower in controls than in patients after IL-10 stim...Continue Reading

References

Feb 1, 1995·Scandinavian Journal of Immunology·V NavikasH Link
Sep 25, 1999·Journal of Neuroimmunology·P Diaz-VillosladaS L Hauser
Nov 24, 1999·Neurology·M E KozovskaJ Z Zhang
May 29, 2000·Immunology Today·B A 't HartJ D Laman
Mar 13, 2001·Annual Review of Immunology·K W MooreA O'Garra
Apr 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M K LevingsM G Roncarolo
Aug 30, 2001·Nature Immunology·L Steinman
Nov 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L YinV K Tuohy
Dec 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lynn WilliamsBrian Foxwell
Dec 31, 2003·Journal of Neuroimmunology·Samantha S SoldanRhonda R Voskuhl
Mar 23, 2004·Annual Review of Immunology·Sidney PestkaPaul B Fisher
Apr 7, 2004·The Journal of Experimental Medicine·Vissia VigliettaDavid A Hafler
Jun 29, 2004·Trends in Pharmacological Sciences·Marco CosentinoGianmario Frigo
Jul 29, 2004·The Journal of Experimental Medicine·Clare Baecher-Allan, David A Hafler
Aug 25, 2004·Trends in Immunology·Rebecca C Riley-VargasJohn P Atkinson
Mar 18, 2005·Annual Review of Immunology·Mireia Sospedra, Roland Martin
Mar 19, 2005·Nature Reviews. Immunology·Jeffrey A Bluestone
Dec 13, 2005·The Journal of Clinical Investigation·Emmanuel XystrakisCatherine M Hawrylowicz
Feb 9, 2006·Seminars in Immunology·Manuela BattagliaMaria-Grazia Roncarolo
Mar 25, 2006·Nature Reviews. Immunology·Weiping Zou
Jun 21, 2006·Annals of Neurology·Beatriz MorenoPablo Villoslada
Jul 28, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Xiao-Song HeHarry B Greenberg
Aug 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tomoki ItoYong-Jun Liu
Sep 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carmen GianfraniMaria-Grazia Roncarolo
Oct 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Grégory MeiffrenMathias Faure
Nov 14, 2006·The Journal of Clinical Investigation·Anne L AstierDavid A Hafler

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Citations

Oct 30, 2009·Archivum Immunologiae Et Therapiae Experimentalis·Gaëlle Le FriecClaudia Kemper
Jan 27, 2011·Archivum Immunologiae Et Therapiae Experimentalis·Siobhan Ni Choileain, Anne L Astier
Oct 15, 2013·The International Journal of Biochemistry & Cell Biology·Hidekazu YamamotoClaudia Kemper
Mar 12, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Herena EixarchJordi Barquinero
Jun 26, 2008·Nature Clinical Practice. Neurology·Alla L Zozulya, Heinz Wiendl
May 5, 2011·Nature Medicine·Margarita Dominguez-VillarDavid A Hafler
Mar 2, 2010·Human Molecular Genetics·Kaushal S GandhiUNKNOWN ANZgene Multiple Sclerosis Genetics Consortium
Dec 4, 2008·The American Journal of Chinese Medicine·Xiaoxia ZhouClaudius U Meyer
Dec 15, 2010·PloS One·Carlos RiverosUNKNOWN ANZgene Multiple Sclerosis Genetics Consortium
Nov 13, 2012·PloS One·Karoline KicklerAnne L Astier
Apr 4, 2014·Journal of Neuroimmunology·Masaaki NiinoSeiji Kikuchi
Dec 29, 2010·Autoimmune Diseases·Kemal Ugur TufekciKursad Genc
Feb 14, 2016·Journal of Neurology·Marc Fakhoury
Aug 8, 2015·Nature Reviews. Immunology·Calliope A DendrouManuel A Friese
Feb 3, 2011·Immunopharmacology and Immunotoxicology·Farhad Jadidi-Niaragh, Abbas Mirshafiey
Nov 14, 2008·Expert Opinion on Pharmacotherapy·Iván Martinez-ForeroPablo Villoslada
Apr 9, 2015·Frontiers in Immunology·Colleen Elizabeth HayesFaye E Nashold
Mar 20, 2015·Immunology and Cell Biology·Lauren CharronAnne L Astier
May 8, 2016·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Imran H MohiuddinNitin J Karandikar
Aug 13, 2014·Multiple Sclerosis : Clinical and Laboratory Research·Ekaterina KotelnikovaPablo Villoslada
Feb 23, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Nadia Ben FredjMahjoub Aouni
Jun 21, 2017·European Journal of Immunology·Klaus G Schmetterer, Winfried F Pickl
Jul 4, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yi-Giien TsaiChing-Yuang Lin
Jan 10, 2018·Cold Spring Harbor Perspectives in Medicine·Alexandra KitzDavid Hafler
Nov 15, 2018·Acta Neurologica Scandinavica·Pernille MelbyeAnnette Bang Oturai
Jan 23, 2019·Journal of Cellular Physiology·Xi JiaJin-An Zhang
Sep 29, 2009·European Journal of Immunology·Anja FuchsClaudia Kemper
Aug 5, 2010·Clinical and Experimental Immunology·J M FletcherK H G Mills
Apr 10, 2014·Immunological Reviews·Markus Kleinewietfeld, David A Hafler
Apr 15, 2011·Clinical and Experimental Immunology·J CardoneC Kemper
May 8, 2015·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Laurine Legroux, Nathalie Arbour
Apr 10, 2010·International Journal of Immunopathology and Pharmacology·I ContastaD Adorno
Sep 13, 2017·Proceedings of the National Academy of Sciences of the United States of America·Kerstin BererHartmut Wekerle
Nov 12, 2019·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Ali J Jahan-AbadAli Gorji
Apr 19, 2020·Journal of Neuroinflammation·Elizabeth M RungeKathryn J Jones
Dec 19, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anne L AstierNir Hacohen
Oct 2, 2015·Clinical & Experimental Neuroimmunology·Elisabeth R MariAbdolmohamad Rostami

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