IL-13 upregulates GPIIb expression in megakaryocytic cell lines via STAT6

The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology
Xiao-yu ShiWen-lin Li

Abstract

Interleukin 13 (IL-13) is a key cytokine involved in the regulation of inflammatory, immune responses, and cell differentiation. The present study was to investigate the effect of IL-13 on the expression of glycoprotein IIb (GPIIb), a megakaryocytic gene, in Dami cells (human megakaryoblastic leukemia cell line) and HEL cells (human erythroleukemic cell line, which has both erythroid and megakaryocytic markers). Furthermore, it addresses the mechanisms governing the regulation of GPIIb expression by IL-13. The molecular responses of Dami cells and HEL cells to IL-13 treatment were analyzed by RT-PCR, Western blot, chromatin immunoprecipitation (ChIP) and flow cytometry analysis. We show that IL-13Rα1 and IL-4Rα are expressed in Dami cells and HEL cells. The expression of GPIIb was significantly upregulated at the mRNA and protein levels by treatment with IL-13. Moreover, IL-13 induced phosphorylation of signal transducer and activator of transcription 6(STAT6). By using a STAT6-specific antibody and PCR primers designed to yield a product, which encompasses the STAT6 binding site of the GPIIb promoter, we have shown the binding of the IL-13-mediated activation of STAT6 to the promoter of GPIIb gene. These results broaden the in...Continue Reading

References

Apr 1, 1990·The Journal of Clinical Investigation·M W LongE V Prochownik
Nov 1, 1988·The Journal of Clinical Investigation·M W LongS G Emerson
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J PunnonenJ E de Vries
Sep 23, 1997·Biochemical and Biophysical Research Communications·T MurataR K Puri
Sep 2, 1998·The Journal of Allergy and Clinical Immunology·J E de Vries
Jan 23, 1999·International Reviews of Immunology·P Chomarat, J Banchereau
Mar 10, 2001·The Journal of Allergy and Clinical Immunology·H C Oettgen, R S Geha
Apr 20, 2002·Cellular and Molecular Life Sciences : CMLS·I P Casserly, E J Topol
Apr 22, 2003·The Journal of Allergy and Clinical Immunology·Gurjit K Khurana Hershey
Jun 7, 2003·Science·Ann E Kelly-WelchAchsah D Keegan
Apr 21, 2004·Drug News & Perspectives·K Izuhara, K Arima
May 4, 2004·Molecular and Cellular Biology·Joel R SevinskyNatalie G Ahn
Oct 7, 2004·The Journal of Biological Chemistry·Pascale JackersDennis K Watson
Nov 18, 2004·Immunological Reviews·Margaret M Mentink-Kane, Thomas A Wynn
Feb 1, 2005·Journal of Virology·Afsar Rahbar, Cecilia Söderberg-Nauclér
May 5, 2005·The Journal of Biological Chemistry·Kazuhiko ArimaKenji Izuhara
Mar 11, 2006·American Journal of Respiratory and Critical Care Medicine·Takako NakanoYoichi Nakanishi
Mar 17, 2006·Cytokine & Growth Factor Reviews·Daniel HebenstreitAlbert Duschl
Feb 26, 2008·Circulation Journal : Official Journal of the Japanese Circulation Society·Yuki NishimuraKoichi Node

❮ Previous
Next ❯

Citations

Jun 1, 2013·In Vitro Cellular & Developmental Biology. Animal·Yazun Bashir JarrarSu-Jun Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.