IL-15 deficiency alleviates steroid-induced osteonecrosis of the femoral head by impact osteoclasts via RANKL-RANK-OPG system

Immunity & Ageing : I & a
Zubin ZhouXiaowei Yu

Abstract

Whether IL-15 is involved in the development of steroid-induced osteonecrosis of the femoral head (ONFH) is investigated. C57BL/6 J and l15-/-mice were injected with methylprednisolone to induce wide type osteonecrosis (WT ON) and IL-15 deficiency osteonecrosis (IL-15-/- ON). Hematoxylin-Eosin (H&E) staining and micro-computed tomography (micro-CT) scanning was used to detect the microstructure. The differentiation and formation of osteoclasts were determined with colony-forming unit-granulocyte macrophages (CFU-GM), colony-forming unit-macrophage/mononuclear (CFU-M) per tibia, and tartrate-resistant acid phosphatase (TRACP or TRAP) positive cells. Serum interleukin (IL)-15, osteocalcin, bone alkaline phosphatase (BAP), bone Gla protein (BGP), and TRACP were assayed with enzyme-linked immunosorbent assay (ELISA). The receptor activator of nuclear factor-κB (RANK), RANK ligand (RANKL), and osteoprotegerin (OPG) in the femoral heads were detected by Western blot. CD34 staining was performed to detect microvascular density. IL-15 secretion was increased in the femoral heads and the serum of steroid-induced ONFH mice. IL-15 deficiency may lead to up-regulated vessel remodeling, improved microstructure, and up-regulated serum osteoc...Continue Reading

References

Mar 3, 1999·Analytical Cellular Pathology : the Journal of the European Society for Analytical Cellular Pathology·P RanefallE Bengtsson
Jul 24, 2004·The Orthopedic Clinics of North America·George C Babis, Panayotis N Soucacos
Mar 24, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M C BezerraR M R Pereira
Sep 2, 2008·The Journal of Bone and Joint Surgery. British Volume·N-F ZhangP Hernigou
Sep 17, 2009·Calcified Tissue International·Jung-Min KohEui Kyun Park
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kalle SöderströmEdgar G Engleman
Aug 4, 2012·Therapeutic Advances in Musculoskeletal Disease·Piet Geusens
Apr 12, 2016·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Toshikazu KuboHiroyoshi Fujiwara
Dec 6, 2016·Journal of Cellular Biochemistry·Hong-Xing ZhaoJin-Gang Tao
May 2, 2017·The Journal of International Medical Research·Qiankun ZhangLie Jin

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ELISA

Software Mentioned

NRecon
CTAn
Data viewer
Cellsense

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
A OnuS Bulfone-Paus
Neuroscience and Biobehavioral Reviews
Weihong PanAbba J Kastin
The Journal of Immunology : Official Journal of the American Association of Immunologists
Y OgataO Kohashi
© 2022 Meta ULC. All rights reserved