IL-17 production elicited by allo-major histocompatibility complex class II recognition depends on CD25posCD4pos T cells

Transplantation
Fleur Samantha BenghiatAlain Le Moine

Abstract

Interleukin (IL)-17 is involved in autoimmune inflammatory disorders and naturally occurring CD25pos regulatory T cells were shown to promote IL-17 synthesis. Because IL-17 is overproduced in certain types of allograft rejection, it is important to characterize the cells responsible for IL-17 synthesis and to define how IL-17 is regulated during alloimmune responses. Splenic CD4pos T cells were isolated from C57BL/6 mice and fractionated according to CD25 expression. T cells were stimulated by major histocompatibility complex class II-mismatched bone marrow-derived dendritic cells from bm12 mice, either immature or made mature by exposure to lipopolysaccharide. To track T cell populations, CD25negCD4pos and CD25posCD4pos were isolated from Thy1.1 and congenic Thy1.2 mice, respectively. Cell proliferation was quantified by CFSE dilution. IL-17-producing cells and FOXP3pos cells were enumerated by intracytoplasmic staining and cytokine levels in culture supernatants were measured by ELISA. Addition of CD25posCD4pos T cells to CD25negCD4pos T cells inhibited IL-2, interferon-[gamma], and IL-13 production but promoted IL-17 synthesis on stimulation by allogenic immature DC. In this setting, IL-17 originated from CD25intCD4posFOXP3n...Continue Reading

References

Jul 31, 2001·Transplantation·J L TangA W Thomson
Jul 13, 2002·The Journal of Pathology·Che-Chuan LoongChing-Yuang Lin
Mar 27, 2003·Nature Reviews. Immunology·Kathryn J Wood, Shimon Sakaguchi
May 20, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Shuiping Jiang, Robert I Lechler
Nov 25, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susumu NakaeYoichiro Iwakura
Dec 10, 2003·The Journal of Experimental Medicine·Craig A MurphyDaniel J Cua
Feb 14, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Atsuhiro OgawaYoshihide Fujiyama
Mar 19, 2005·Nature Reviews. Immunology·Jeffrey A Bluestone
Apr 6, 2006·The European Respiratory Journal·B M VanaudenaerdeG M Verleden
Jul 25, 2006·Transplantation·Matthew O BrookNick D Jones
Sep 16, 2006·Immunological Reviews·Stephen P CobboldHerman Waldmann
Nov 30, 2006·The Journal of Experimental Medicine·Jens LohrAbul K Abbas
Jan 19, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S Goriely, M Goldman
Jul 27, 2007·Nature Reviews. Immunology·Maria-Grazia Roncarolo, Manuela Battaglia

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Citations

Jul 10, 2010·Current Opinion in Organ Transplantation·Sebastiaan HeidtKathryn J Wood
Dec 22, 2011·Current Opinion in Organ Transplantation·Farida AbadjaMohammed J Ansari
Dec 7, 2011·Circulation·Koichi ShimizuKevin Croce
Jun 30, 2009·Experimental & Molecular Medicine·Sang Il MinSang Joon Kim
May 19, 2009·Current Opinion in Organ Transplantation·Peter MitchellRobert I Lechler
May 7, 2009·Current Opinion in Organ Transplantation·Peter NickelPetra Reinke
Oct 28, 2009·Transplantation Proceedings·A Le MoineL M Charbonnier
Nov 26, 2008·Transplantation Reviews·Fleur Samantha BenghiatAlain Le Moine
Mar 3, 2009·Clinical and Experimental Immunology·M Goldman, K Wood
Feb 15, 2013·Immunological Reviews·Zhongmin LiuShuiping Jiang
Aug 25, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Benoît VokaerLouis-Marie Charbonnier
Jun 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yoshito YamadaDavid S Wilkes
Feb 5, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Scott M KrummeyMandy L Ford

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