IL-17A+ GM-CSF+ Neutrophils Are the Major Infiltrating Cells in Interstitial Lung Disease in an Autoimmune Arthritis Model

Frontiers in Immunology
Oh Chan KwonYong-Gil Kim

Abstract

To gain a better understanding of the pathogenesis of autoimmune arthritis-associated interstitial lung disease (ILD), we sought to identify the characteristics of lung-infiltrating cells in SKG mice with ILD. We injected curdlan in SKG mice at 8 weeks of age, and identified the presence of ILD by PET-MRI at 20 weeks post-injection and histological analysis at 22 weeks post-injection. Lung-infiltrating cells were examined by flow cytometry. Analysis of serum cytokines by the Luminex multiplex cytokine assay was performed at 14 and 22 weeks post-injection, and cytokine profiles before and after the development of ILD were compared. Opal multiplexed immunofluorescent staining of lung tissue was also performed. At 20 weeks post-injection, curdlan-treated SKG mice developed not only arthritis but also lung inflammation combined with fibrosis, which was identified by PET-MRI and histological analysis. The majority of inflammatory cells that accumulated in the lungs of curdlan-treated SKG mice were CD11b+Gr1+ neutrophils, which co-express IL-17A and GM-CSF, rather than TNF-α. Compared with 14 weeks post-injection, serum levels of GM-CSF, MCP1, IL-17A, IL-23, TSLP, and soluble IL-7Rα had increased at 22 weeks post-injection, whereas t...Continue Reading

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Citations

Oct 16, 2019·The Journal of Experimental Medicine·John A Hamilton
Feb 3, 2019·Immunology and Cell Biology·Tiffany Bouchery, Nicola Harris
Jun 25, 2020·Nature Reviews. Immunology·Frederick M LangJohn A Hamilton
Nov 6, 2020·ImmunoTargets and Therapy·Kevin M C LeeJohn A Hamilton

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
Flow Cytometry
bronchoalveolar lavage

Software Mentioned

GraphPad Prism
GraphPad

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