IL-17F/IL-17R interaction stimulates granulopoiesis in mice

Experimental Hematology
Weihong TanPaul Schwarzenberger

Abstract

IL-17F, a member of the interleukin (IL)-17 cytokine family, most closely resembles IL-17A structurally. IL-17A is a potent stimulator of granulopoiesis; its expression is induced in response to microbial challenge. Although IL-17F is considered to be a weak IL-17A analog that is also mediating its effect via IL-17R, its exact role and in vivo functions are unknown. Our goal was to determine the in vivo activity of IL-17F on granulopoiesis as well as on release of granulopoiesis-stimulating downstream cytokines in mice and directly compare its effect to IL-17A. Murine IL-17A (mIL-17A) or IL-17F (mIL-17F) was expressed in vivo in C57BL6 mice using adenoviral gene transfer technology. Peripheral cell counts were assessed as well as hematopoietic precursors using colony-forming assays at set time points. Downstream cytokines were measured using enzyme-linked immunosorbent assay and reverse transcriptase polymerase chain reaction. We found mIL-17F to have similar expression kinetics as mIL-17A in splenocytes in vitro and in vivo, following challenge with microbial agents. Overexpression of mIL-17F in vivo resulted in similar neutrophilia and only in slightly reduced myeloid progenitor expansion when compared to mIL-17A. In vivo, th...Continue Reading

Citations

Oct 12, 2012·Journal of Cardiovascular Translational Research·Slava Epelman, Douglas L Mann
Jun 30, 2010·Infection and Immunity·Louise HenningssonInger Gjertsson
Apr 9, 2010·Kidney International·Sibylle von VietinghoffKlaus Ley
Aug 26, 2014·Experimental Cell Research·Cristina MirantesEric M Pietras
Jan 24, 2015·Nature Reviews. Immunology·Slava EpelmanDouglas L Mann
Jun 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sibylle von Vietinghoff, Klaus Ley
Jan 16, 2009·Experimental Biology and Medicine·Hong LinSusanna Cunningham-Rundles
Feb 9, 2021·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Anja BecherRomy M Heilmann

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