IL-18 acts in synergy with IL-7 to promote ex vivo expansion of T lymphoid progenitor cells

The Journal of Immunology : Official Journal of the American Association of Immunologists
Siva K GandhapudiT Kent Teague

Abstract

Although IL-18 has not previously been shown to promote T lymphopoiesis, results obtained via a novel data mining algorithm (global microarray meta-analysis) led us to explore a predicted role for this cytokine in T cell development. IL-18 is a member of the IL-1 cytokine family that has been extensively characterized as a mediator of inflammatory immune responses. To assess a potential role for IL-18 in T cell development, we sort-purified mouse bone marrow-derived common lymphoid progenitor cells, early thymic progenitors (ETPs), and double-negative 2 thymocytes and cultured these populations on OP9-Delta-like 4 stromal layers in the presence or absence of IL-18 and/or IL-7. After 1 wk of culture, IL-18 promoted proliferation and accelerated differentiation of ETPs to the double-negative 3 stage, similar in efficiency to IL-7. IL-18 showed synergy with IL-7 and enhanced proliferation of both the thymus-derived progenitor cells and the bone marrow-derived common lymphoid progenitor cells. The synergistic effect on the ETP population was further characterized and found to correlate with increased surface expression of c-Kit and IL-7 receptors on the IL-18-treated cells. In summary, we successfully validated the global microarra...Continue Reading

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Citations

Sep 25, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Veronica BordoniFederico Martini
Dec 10, 2019·The Journal of Experimental Medicine·Maryam GhaediFumio Takei
Jul 26, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Magali M MorettoImtiaz A Khan
Mar 29, 2018·Cellular and Molecular Life Sciences : CMLS·Chao HuangXin A Zhang
Jul 16, 2021·Journal of Extracellular Vesicles·Balaji KrishnamacharyNavneet K Dhillon

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