IL-1alpha and IL-1beta have different effects on formation and activity of large osteoclasts

Journal of Cellular Biochemistry
Diana P Trebec-ReynoldsMorris F Manolson

Abstract

Interleukin 1 (IL-1) is a proinflammatory cytokine upregulated in conditions such as rheumatoid arthritis and periodontal disease. Both isoforms, IL-1alpha and IL-1beta, have been shown to activate osteoclasts (OCs), the cells responsible for resorbing bone. Inflammatory conditions are also characterized by increased bone loss and by the presence of large OCs (10+ nuclei). We and others have previously shown that large OCs are more likely to be resorbing compared to small OCs (2-5 nuclei). Moreover, large OCs express higher levels of the IL-1 activating receptor IL-1RI, integrins alphav and beta3, RANK, and TNFR1, while small OCs have higher levels of the decoy receptor IL-1RII. We hypothesized that IL-1 would have different effects on large and small OCs due to these distinct receptor expression patterns. To test this hypothesis, RAW 264.7 cells were differentiated into populations of small and large OCs and treated with IL-1alpha or IL-1beta (1 and 10 ng/ml). In the presence of sRANKL, both IL-1alpha and IL-1beta increased total OC number and resorptive activity of large OCs. IL-1alpha stimulated formation of large OCs and increased the number of resorption pits, while IL-1beta changed the morphology of large OCs and integrin...Continue Reading

References

Aug 1, 1991·British Journal of Rheumatology·J A EastgateG W Duff
Jul 1, 1994·Clinical Immunology and Immunopathology·J E SimsS K Dower
Feb 1, 1996·Microscopy Research and Technique·P T Lakkakorpi, H K Väänänen
Aug 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R Pacifici
Dec 1, 1996·Journal of Periodontology·C Kanda-NakamuraT Sueda
Jul 1, 1998·International Reviews of Immunology·C A Dinarello
Sep 22, 1998·Scandinavian Journal of Immunology·H LettesjöE Möller
Jun 8, 1999·The Journal of Clinical Investigation·R SinghJ R Sedor
Jul 31, 1999·Experimental Hematology·G D Roodman
Feb 16, 2000·Journal of Clinical Periodontology·L RasmussenU H Lerner
Jun 8, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·L T DuongG A Rodan
Dec 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaoping SongRon N Apte
Feb 6, 2004·Journal of Electron Microscopy·Ichiro NakamuraLe T Duong
May 20, 2004·Rheumatology·V Strand, A F Kavanaugh
Sep 17, 2004·Molecular Biology of the Cell·Frédéric SaltelPierre Jurdic
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Benjamin GeigerAlexander D Bershadsky
Sep 16, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yaron CarmiRon N Apte

❮ Previous
Next ❯

Citations

Nov 2, 2011·Internal and Emergency Medicine·Gianfranco Ferraccioli, Elisa Gremese

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.