IL-1beta activates p44/42 and p38 mitogen-activated protein kinases via different pathways in cat esophageal smooth muscle cells

World Journal of Gastroenterology : WJG
Tai Sang LeeUy Dong Sohn

Abstract

To examine the pathway related to the IL-1beta-induced activation of mitogen-activated protein (MAP) kinases in cat esophageal smooth muscle cells. Culture of the esophageal smooth muscle cells from cat was prepared. Specific inhibitors were treated before applying the IL-1beta. Western blot analysis was performed to detect the expressions of COX, iNOS and MAP kinases. In the primary cultured cells, although IL-1beta failed to upregulate the COX and iNOS levels, the levels of the phosphorylated forms of p44/42 MAP kinase and p38 MAP kinase increased in both concentration- and time-dependent manner, of which the level of activation reached a maximum within 3 and 18 h, respectively. The pertussis toxin reduced the level of p44/42 MAP kinase phosphorylation. Tyrphostin 51 and genistein also inhibited this activation. Neomycin decreased the density of the p44/42 MAP kinase band to the basal level. Phosphokinase C (PKC) was found to play a mediating role in the IL-1beta-induced p44/42 MAP kinase activity. In contrast, the activation of p38 MAP kinase was inhibited only by a pretreatment with forskolin, and was unaffected by the other compounds. Based on these results, IL-1beta-induced p44/42 MAP kinase activation is mediated by the ...Continue Reading

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Citations

Jan 6, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Arindam SahaAdam J Smolka
Oct 29, 2009·Alcoholism, Clinical and Experimental Research·Chao WangChuanyong Liu
Mar 22, 2014·PloS One·Jeffrey S OtisSimon J Lees

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