IL-1beta as a determinant in silica-induced cytokine responses in monocyte-endothelial cell co-cultures

Human & Experimental Toxicology
Ji HersethPe Schwarze

Abstract

Alveolar macrophages and endothelial cells are both involved in lung inflammation and remodeling of lung alveolar structures. In the present study, monocytes (precursors for macrophages) were exposed to crystalline silica and examined for pro- and anti-inflammatory cytokine responses in non-contact co-cultures with endothelial cells. The time courses for silica-induced release of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-8 both from co-cultures and monocyte mono-cultures showed an early peak at 5-10 h, almost no response at 20 h, and a strong increase at 43 h. At 43 h, co-cultures also showed strongly increased IL-6 levels. Steady-state levels of mRNA roughly exhibited the same pattern of early up-regulation and reduced levels at 20 h. Compared with monocyte mono-cultures, silica induced a strong release of IL-1beta, IL-6, and IL-8, but not of TNF-alpha, after 43 h in co-cultures, whereas at 5 and 10 h a significant difference was only observed for the silica-induced IL-8 response. An antagonist to the IL-1 receptor strongly reduced IL-6 and IL-8 levels, whereas antibodies to TNF-alpha increased the levels of IL-1beta and IL-8. Thus, IL-1beta is suggested to be an important triggering factor that determi...Continue Reading

References

Jul 1, 1993·The American Review of Respiratory Disease·A D OxmanH J Lange
Jun 1, 1996·Clinical Immunology and Immunopathology·I ShaharS Ben Efraim
Dec 24, 1997·Environmental Health Perspectives·K E DriscollB Howard
Oct 13, 1998·The Journal of Bone and Joint Surgery. British Volume·M LindR L Smith
Dec 16, 1998·American Journal of Respiratory and Critical Care Medicine·J DaiA Churg
Apr 18, 2000·Chest·S M Opal, V A DePalo
Aug 11, 2000·Chest·C A Dinarello
Sep 26, 2000·Human & Experimental Toxicology·G HetlandA Aase
Mar 29, 2002·American Journal of Respiratory Cell and Molecular Biology·Florence Tao, Lester Kobzik
Jul 2, 2002·American Journal of Respiratory Cell and Molecular Biology·Takeshi FujiiStephan F van Eeden
Oct 12, 2002·Paediatric Respiratory Reviews·John W Wilson, Colin F Robertson
Dec 7, 2002·Life Sciences·Sergei P Atamas
Apr 18, 2003·Cardiovascular Radiation Medicine·Rajbabu PakalaClaude R Benedict
Oct 18, 2003·Cytokine & Growth Factor Reviews·Peter J Barnes
Dec 19, 2003·Biochemical and Biophysical Research Communications·Katsuhisa OshikawaYukihiko Sugiyama
Jan 20, 2004·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·A MoroguchiH Maeta
Jan 30, 2004·Annual Review of Medicine·Victor J ThannickalFernando J Martinez
Feb 3, 2004·American Journal of Respiratory and Critical Care Medicine·Masahito EbinaToshihiro Nukiwa
Feb 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mitchell A OlmanMichael A Matthay
Mar 9, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hiroshi IshiiStephan F van Eeden
Apr 13, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Maria Iris HermannsCharles James Kirkpatrick
Jun 8, 2004·Current Drug Targets. Inflammation and Allergy·Kamran Ghoreschi, Martin Röcken
Aug 10, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A Di StefanoC F Donner
Oct 9, 2004·International Journal of Hygiene and Environmental Health·Ralf WottrichHarald F Krug
Dec 8, 2004·Environmental Health Perspectives·K Murali Krishna RaoVince Castranova
Feb 5, 2005·Alimentary Pharmacology & Therapeutics·C ShenJ L Ceuppens
Apr 9, 2005·Particle and Fibre Toxicology·Miriam E Gerlofs-NijlandFlemming R Cassee
Jun 1, 2005·The American Journal of Cardiology·Anthony E Bolton
Mar 2, 2006·Environmental Health Perspectives·Timothy R NurkiewiczMatthew A Boegehold

❮ Previous
Next ❯

Citations

Jul 8, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jan I HersethPer E Schwarze
Jan 17, 2016·Annual Review of Immunology·Bernardo S FranklinEicke Latz
Oct 3, 2020·Inhalation Toxicology·Kaio Cezar Rodrigues SalumFabiana Barzotto Kohlrausch
Dec 4, 2010·Particle and Fibre Toxicology·Dorota NapierskaPeter H Hoet

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.