IL-1beta regulation of BDNF expression in rat cultured hypothalamic neurons depends on the presence of glial cells

Neurochemistry International
Florence RageLucia Tapia-Arancibia

Abstract

In the present study, we have shown that IL-1beta increased BDNF mRNA expression in hypothalamic neuron-enriched cultures whereas it reduced this expression in mixed cultures, i.e. containing astrocytes and neurons. Because functional relationships between stress and immunity signals are well documented we investigated the possible interaction between BDNF and IL-1beta in hypothalamic neurons. Notably, we investigated whether IL-1beta affected BDNF expression in vitro either on hypothalamic mixed cultures or on neuron-enriched cultures. We found that the response to IL-1beta was stimulatory when directly examined in neurons but was inhibitory when astrocytes were present in the cultures. Since it has been documented that astrocytes release PGE2 in response to IL-1beta, we examined the effect of indomethacin (a PGE2 synthesis inhibitor) on mixed or neuron-enriched cultures treated with IL-1beta. Indomethacin blocked both stimulatory and inhibitory IL-1beta effects on BDNF mRNA expression whereas picrotoxin (a GABA(A) blocker) or MK-801 (a NMDA receptor blocker) had no effect on BDNF mRNA levels. About 3 and 6h treatments of cells with exogenous PGE2 reproduced the effects of IL-1beta on neuron-enriched or on mixed cultures sugge...Continue Reading

References

Feb 1, 1997·European Journal of Biochemistry·M K BauerB L Fiebich
Aug 11, 1998·Neuroimmunomodulation·C FelederJ A Moguilevsky
Apr 15, 1999·Annual Review of Neuroscience·A K McAllisterD C Lo
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A BacciM Matteoli
Jun 8, 2000·The Journal of Comparative Neurology·K NakamuraM Negishi
Aug 5, 2000·Brain Research. Molecular Brain Research·L C ParkerG N Luheshi
Dec 18, 2001·Journal of Neuroscience Research·F MarmigèreL Tapia-Arancibia
Oct 27, 2004·The Veterinary Journal·Rosemary M GibsonRos A Le Feuvre
Nov 3, 2004·Molecular and Cellular Neurosciences·Laurent GivaloisLucia Tapia-Arancibia
Mar 2, 2005·Nature Reviews. Immunology·Ronald Glaser, Janice K Kiecolt-Glaser

❮ Previous
Next ❯

Citations

Apr 15, 2009·Molecular Neurobiology·Amy M Hein, M Kerry O'Banion
Apr 24, 2012·Expert Opinion on Therapeutic Targets·Weiya MaPedro Cruz Duarte
Dec 31, 2008·Journal of Cellular Physiology·Silvia Di LoretoFernanda Amicarelli
Oct 3, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·S KindtJ Tack
Jan 30, 2010·The World Journal of Biological Psychiatry : the Official Journal of the World Federation of Societies of Biological Psychiatry·Atsuko Ikenouchi-SugitaJun Nakamura
Aug 8, 2007·Neurochemistry International·Damijana Mojca JuricMarija Carman-Krzan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.