IL-2 and IL-10 production by human CD4+T cells is differentially regulated by p38: mode of stimulation-dependent regulation of IL-2

Neuroimmunomodulation
Christina VeiopoulouGeorge Thyphronitis

Abstract

Antigenic stimulation of T cells initiates a complex series of intracellular signaling pathways that target and activate different cytokine genes. The participation of mitogen-activated protein kinases (MAPKs) in these processes has not been studied thoroughly and in some instances conflicting results have been reported. Here we have examined the role of p38 MAPK on IL-2 and IL-10 production following activation of human CD4+ T cells or of the leukemic cell line Hut-78, with either plate-bound anti-CD3 in the presence or absence of soluble anti-CD28 (plCD3, plCD3/sCD28), or with cross-linked anti-CD3 and anti-CD28 (crsCD3+CD28), or with PMA plus ionomycin. Pharmacological inhibition of the p38 pathway with either SB203580, SB202190, or SKF86002 strongly downregulated IL-10 production by T cells stimulated with any of the above treatments. In contrast the effect of p38 inhibition on IL-2 was stimulus dependent. Thus, p38 inhibition strongly upregulated IL-2 production (up to 10-fold) in the plCD3- and plCD3/sCD28-stimulated cultures while it had minimal or no effect in the other two stimulation protocols. Intracellular and mRNA levels of IL-2 and IL-10 were also upregulated and downregulated, respectively, by p38 inhibitors in t...Continue Reading

Citations

Aug 21, 2010·Immunologic Research·Robert L Furler, Christel H Uittenbogaart
Mar 18, 2006·Arthritis Research & Therapy·Francis Dodeller, Hendrik Schulze-Koops
Aug 4, 2010·Cellular and Molecular Life Sciences : CMLS·Elisavet SertiUrania Georgopoulou
Oct 16, 2015·European Journal of Immunology·Sebastián A RiquelmeAlexis M Kalergis
Feb 5, 2008·Journal of Dermatological Science·Dvora Sredni-KenigsbuchMichael David
May 5, 2010·Biochemical and Biophysical Research Communications·Seung-Chul ChoiJae Wha Kim
Feb 16, 2006·Journal of Leukocyte Biology·Olga KogkopoulouGeorge Thyphronitis
Jun 3, 2010·American Journal of Physiology. Endocrinology and Metabolism·Michele D'EliaJacques Bernier
Sep 26, 2020·Nature Communications·Maria Angelica CortezJames W Welsh
Nov 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michelle MelinoAntonio Ferrante

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