IL-2 Inhibition of Th17 Generation Rather Than Induction of Treg Cells Is Impaired in Primary Sjögren's Syndrome Patients

Frontiers in Immunology
Jing LuoLingli Dong

Abstract

To investigate the role of IL-2 in the balance of Th17 and Tregs and elucidate the underlying mechanisms of enhanced Th17 differentiation in primary Sjögren's syndrome (pSS) patients. This study involved 31 pSS patients, 7 Sicca patients, and 31 healthy subjects. Th17 and Treg cells were determined by flow cytometry, and IL-17A was detected by immunohistochemistry. IL-2 and IL-6 levels were assessed by ELISA and qPCR. p-STAT5 and p-STAT3 in salivary glands (SGs) were evaluated by immunohistochemistry and flow cytometry. The binding of STAT5 and STAT3 to the Il17a gene locus was measured by chromatin immunoprecipitation. We found that the percentage of Th17 cells was increased in the periphery and SG of pSS patients when compared with healthy subjects, but the Treg cells was unchanged. Meanwhile, the IL-2 level was reduced, and the IL-6 and IL-17A level was increased in the plasma of pSS patients. The ratio of IL-2 and IL-6 level was also decreased and IL-2 level was negatively correlated with the level of IL-17A. The expression of Il6 and Il17a mRNA was significantly increased, whereas Foxp3, Tgfb1, Tnfa, and Ifng mRNA were comparable. Furthermore, the level of STAT5 phosphorylation (p-STAT5) was reduced and p-STAT3 was enhance...Continue Reading

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Citations

Nov 15, 2018·Modern Rheumatology·Toshio Odani, John A Chiorini
Apr 24, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael L RidleyLeonie S Taams
Sep 11, 2019·Investigative Ophthalmology & Visual Science·Mingli QuQingjun Zhou
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Dec 19, 2019·Frontiers in Immunology·Tingting DingCaihong Wang
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Nov 4, 2021·Nature Reviews. Rheumatology·Antonios G A KoliosDavid Klatzmann

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Methods Mentioned

BETA
density-gradient centrifugation
biopsy
fluorescence imaging
enzyme-linked immunosorbent assay
flow cytometry
ELISA

Software Mentioned

Flow Jo

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